A rolling circle amplification based platform for ultrasensitive detection of heparin.
A rolling circle amplification based platform for ultrasensitive detection of heparin.
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基于滚环扩增的超灵敏检测肝素的平台。
DOI：
10.1039/d0an02061c
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发表时间：
2021-01-21
期刊：
影响因子：
--
通讯作者：
Linhardt RJ
中科院分区：
文献类型：
--
作者：
Lin L ;Li B ;Han X ;Zhang F ;Zhang X ;Linhardt RJ
肝素具有多种药理学用途，包括用于抗肿瘤转移、抗炎和抗病毒活性的应用，并且广泛用作临床抗凝剂。由于其在临床程序中的广泛应用，监测肝素水平对于确保肝素的安全使用以及防止过量和并发症（如出血和血小板减少症）至关重要。然而，传统的肝素检测依赖于活化凝血时间或活化部分促凝血酶原激酶时间的测量，这对于某些临床环境来说不是足够可靠或准确的测量。在这项工作中，我们描述了一种哑铃探针辅助策略，用于基于独特的强鱼精蛋白-肝素相互作用和滚环放大驱动的信号放大的肝素的超灵敏和等温检测。肝素的检测限为12.5 ng mL−1（0.83 nM），远低于心血管手术中肝素的治疗水平（17-67 μM）以及术后和长期治疗中肝素的治疗水平（1.7-10 μM）。此外，所提出的传感平台对于人血浆样品中的肝素监测工作良好。这种结构简单、灵敏度高的肝素生物传感器在诊断、治疗和生物学研究中具有潜在的应用价值。用于超灵敏肝素分析的哑铃探针辅助RCA传感平台。
Heparin has a variety of pharmacological uses, including applications for anti-tumor metastasis, anti-inflammatory and anti-viral activities and is widely used as a clinical anticoagulant. Due to its widespread applications in the clinical procedures, monitoring heparin levels is critically important to ensure the safe use of heparin and to prevent overdose and complications, such as hemorrhage and thrombocytopenia. However, traditional heparin detection relies on the measurements of the activated clotting time or activated partial thromboplastin time, which are not sufficiently reliable or accurate measurements for certain clinical settings. In this work, we describe a dumbbell probe-aided strategy for ultrasensitive and isothermal detection of heparin based on a uniquely strong protamine-heparin interaction and rolling circle amplification driven signal amplification. The detection limit for heparin is 12.5 ng mL−1 (0.83 nM), which is much lower than the therapeutic level of heparin in cardiovascular surgery (17–67 μM) and in postoperative and long-term treatment (1.7–10 μM). Additionally, the proposed sensing platform works well for heparin monitoring in human plasma samples. This simple and ultrasensitive heparin biosensor has potential application in diagnostics, therapeutics, and in biological research. A dumbbell probe-aided RCA sensing platform for ultrasensitive heparin analysis.
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影响因子：
15
作者：
Shvarev, A;Bakker, E
通讯作者：
Bakker, E
DOI：
10.1073/pnas.1902227116
发表时间：
2019-05-07
影响因子：
11.1
作者：
Zhang, Xing;Han, Xiaorui;Linhardt, Robert J.
通讯作者：
Linhardt, Robert J.
影响因子：
7.4
作者：
Meyerhoff, ME;Fu, B;Yang, VC
通讯作者：
Yang, VC
影响因子：
11.9
作者：
Liu J;Linhardt RJ
通讯作者：
Linhardt RJ
影响因子：
4.9
作者：
Choi, Justin M.;Bourassa, Valerie;Udit, Andrew K.
通讯作者：
Udit, Andrew K.