Variovorax durovernensis sp. nov., a novel species isolated from an infected prosthetic aortic graft in a human.
Variovorax durovernensis sp. nov., a novel species isolated from an infected prosthetic aortic graft in a human.
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杜罗韦尔瓦里奥沃拉克斯 (Variovorax durovernensis)
DOI：
10.1099/ijsem.0.006184
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发表时间：
2023
影响因子：
2.8
通讯作者：
Alcolea-Medina A
中科院分区：
文献类型：
--
作者：
Alcolea-Medina A
一株新的细菌菌株GSTT-20T是从英国伦敦的一只牧羊犬身上移植的感染的人造血管内移植物上分离出来的。该菌株是一株需氧、过氧化氢酶阳性、氧化酶阴性、革兰氏染色阴性、能动的弯曲杆菌。在37 °C、有氧环境中培养48 h后，在血液琼脂、巧克力琼脂和麦康基琼脂上生长，形成黄色粘液状菌落。对16S rRNA基因全序列的分析表明，该基因与Variovorax paroxus和Variovorax boronicumulans的同源性分别为99.6 %和99.5 %。16SRRNA基因序列的系统发育分析和1530个核心基因的单核苷酸多态性的系统发育分析表明，GSTT-20T在菊科变种基因组中形成了一个明显的谱系。B、V. %为31.9%。悖论。与GSTT-20TtoV比较，基于平均核苷酸同源性的基因组相似性为87.50 %。悖论。根据这些结果，该菌株代表了一个新的种，命名为Variovorax durovernensssp.11月是被提议的。模式菌株为GSTT-20T(NCTC 14621T=CECT 30390T)。
A novel bacterial strain, GSTT-20Twas isolated from an infected, prosthetic endovascular graft explanted from a shepherd in London, United Kingdom. This strain was an aerobic, catalase-positive, oxidase-negative, Gram-stain-negative, motile, curved rod. It grew on blood agar, chocolate agar and MacConkey agar incubated at 37 °C in an aerobic environment after 48 h, appearing as yellow, mucoid colonies. Analysis of the complete 16S rRNA gene sequence showed closest similarity toVariovorax paradoxuswith 99.6 % identity andVariovorax boronicumulanswith 99.5 % identity. Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of single nucleotide polymorphisms within 1530 core genes showed GSTT-20Tforms a distinct lineage in the genusVariovoraxof the familyComamonadaceae.In silicoDNA–DNA hybridization assays against GSTT-20Twere estimated at 32.1 % forV. boronicumulansand 31.9 % forV. paradoxus. Genome similarity based on average nucleotide identity was 87.50 % when comparing GSTT-20TtoV. paradoxus. Based on these results, the strain represented a novel species for which the nameVariovorax durovernensissp. nov. was proposed. The type strain is GSTT-20T(NCTC 14621T=CECT 30390T).
影响因子：
46.9
作者：
Kolmogorov, Mikhail;Yuan, Jeffrey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
对90K核基因组的高通量ANI分析揭示了清晰的物种边界。
影响因子：
16.6
作者：
Jain C;Rodriguez-R LM;Phillippy AM;Konstantinidis KT;Aluru S
通讯作者：
Aluru S
DOI：
10.1007/s10482-014-0304-3
发表时间：
2015-01-01
影响因子：
2.6
作者：
Gao, Jun-lian;Yuan, Mei;Sun, Jian-guang
通讯作者：
Sun, Jian-guang