Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against Hessian fly.
Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against Hessian fly.
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DOI：
10.1186/1471-2164-14-423
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发表时间：
2013-06-26
期刊：
影响因子：
4.4
通讯作者：
Chen MS
中科院分区：
文献类型：
--
作者：
Khajuria C;Wang H;Liu X;Wheeler S;Reese JC;El Bouhssini M;Whitworth RJ;Chen MS
小麦-黑森蝇的互作遵循典型的基因对基因模型。黑森蝇幼虫在携带有效抗性基因的小麦植株中死亡，或者在不携带有效抗性基因的易感植株中茁壮成长。在抗性植物中受黑森蝇攻击影响的基因组被发现与易感植物中的基因组有很大不同。基因组的差异表达与防御途径中中间产物的差异积累有关。我们的研究结果表明，资源被迅速动员在抗性植物的防御，包括广泛的膜重塑和释放的脂质，糖催化剂，氨基酸的运输和降解。这些资源可能迅速转化为防御分子，如氧脂;有毒蛋白质，包括半胱氨酸蛋白酶，消化酶抑制剂和凝集素;酚类化合物;和细胞壁成分。然而，毒性本身不会对黑森蝇幼虫造成立即致死。有毒的防御可能会减缓黑森蝇的发育，因此给植物更多的时间让其他类型的防御发挥作用。我们的基因表达和代谢分析结果表明，重塑和强化细胞壁和角质层的增加沉积的酚类化合物和增强交联可能是至关重要的昆虫死亡率剥夺黑森蝇幼虫的营养物质从宿主细胞。大量在亲和和不亲和互作过程中不同时间点差异表达基因的鉴定也为进一步研究导致小麦对黑森蝇侵染的抗性和易感性的分子途径提供了基础。
Wheat – Hessian fly interaction follows a typical gene-for-gene model. Hessian fly larvae die in wheat plants carrying an effective resistance gene, or thrive in susceptible plants that carry no effective resistance gene. Gene sets affected by Hessian fly attack in resistant plants were found to be very different from those in susceptible plants. Differential expression of gene sets was associated with differential accumulation of intermediates in defense pathways. Our results indicated that resources were rapidly mobilized in resistant plants for defense, including extensive membrane remodeling and release of lipids, sugar catabolism, and amino acid transport and degradation. These resources were likely rapidly converted into defense molecules such as oxylipins; toxic proteins including cysteine proteases, inhibitors of digestive enzymes, and lectins; phenolics; and cell wall components. However, toxicity alone does not cause immediate lethality to Hessian fly larvae. Toxic defenses might slow down Hessian fly development and therefore give plants more time for other types of defense to become effective. Our gene expression and metabolic profiling results suggested that remodeling and fortification of cell wall and cuticle by increased deposition of phenolics and enhanced cross-linking were likely to be crucial for insect mortality by depriving Hessian fly larvae of nutrients from host cells. The identification of a large number of genes that were differentially expressed at different time points during compatible and incompatible interactions also provided a foundation for further research on the molecular pathways that lead to wheat resistance and susceptibility to Hessian fly infestation.
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影响因子：
6.9
作者：
Bolwell, GP;Bindschedler, LV;Minibayeva, F
通讯作者：
Minibayeva, F
影响因子：
2.3
作者：
Causton, CE;Peck, SB;Landry, B
通讯作者：
Landry, B
影响因子：
7.2
作者：
Konno, K;Hirayama, C;Kohno, K
通讯作者：
Kohno, K
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.5
作者：
Bodenhausen, Natacha;Reymond, Philippe
通讯作者：
Reymond, Philippe