Stromal cell-derived factor-1 and its receptor CXCR4 are upregulated expression in degenerated intervertebral discs.
Stromal cell-derived factor-1 and its receptor CXCR4 are upregulated expression in degenerated intervertebral discs.
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基质细胞衍生因子 1 及其受体 CXCR4 在退变椎间盘中表达上调
DOI：
10.7150/ijms.7489
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发表时间：
2014
影响因子：
3.6
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Zhang H;Zhang L;Chen L;Li W;Li F;Chen Q
背景资料：尽管趋化因子基质细胞衍生因子1（SDF-1）及其受体CXCR 4诱导类风湿性关节炎（RA）和骨关节炎（OA）中关节软骨的降解，但SDF-1/CXCR 4通路与软骨终板和髓核降解之间的关联尚未完全阐明。我们研究了SDF-1和CXCR 4在椎间盘（IVD）中的表达。方法：采用酶联免疫吸附法（ELISA）检测人IVD和大鼠L5/6运动节段中SDF-1和CXCR 4的表达。使用显微镜和Image-Pro Plus软件定量SDF-1染色。积分光密度（IOD）作为测量参数。CXCR 4免疫反应性细胞的数量表示为细胞总数的百分比。结果如下：SDF-1和CXCR 4在椎间盘退变中均有表达，退变组SDF-1和CXCR 4的表达水平均显著高于正常组。髓核细胞和软骨终板细胞均表达CXCR 4蛋白。此外，SDF-1 IOD值与髓核和软骨终板中CXCR 4阳性椎间盘细胞的百分比呈正相关。在大鼠标本中，外纤维环和骨/终板连接区域的SDF-1 IOD值显着高于髓核和软骨终板。结论：SDF-1及其受体CXCR 4在退行性IVD中表达上调。
Background: Although chemokine stromal cell-derived factor 1 (SDF-1) and its receptor CXCR4 induce degradation of articular cartilage in rheumatoid arthritis (RA) and osteoarthritis (OA), the association between the SDF-1/CXCR4 pathway and degradation of the cartilaginous endplate and nucleus pulposus has not been thoroughly clarified. We investigated the expression of SDF-1 and CXCR4 in intervertebral discs (IVDs). Methods: SDF-1 and CXCR4 levels in human IVDs and the rat L5/6 motion segment were quantified by enzyme-linked immunosorbent assay. SDF-1 staining was quantified using a microscope and Image-Pro Plus software. Integrated optical density (IOD) served as the measurement parameter. The number of CXCR4 immunoreactive cells was expressed as a percentage of the total number of cells. Results: SDF-1 and CXCR4 were both expressed in IVDs, and the levels of SDF-1 and CXCR4 were both significantly higher in the degeneration group than in the normal group of human (or rat) discs. Both nucleus pulposus cells and cartilaginous endplate cells expressed the CXCR4 protein. Furthermore, a positive correlation was observed between the SDF-1 IOD value and the percentage of CXCR4-positive disc cells in the nucleus pulposus and cartilaginous endplate. The SDF-1 IOD values were significantly higher in the outer annular fibrosus and bone/endplate junction region than in the nucleus pulposus and cartilaginous endplate in the rat specimens. Conclusions: Our findings suggest upregulated expression of SDF-1 and its receptor CXCR4 in degenerated IVD.
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1.6
作者：
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