Expression and regulation of activated leukocyte cell adhesion molecule in human retinal vascular endothelial cells.
Expression and regulation of activated leukocyte cell adhesion molecule in human retinal vascular endothelial cells.
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DOI：
10.1016/j.exer.2012.08.006
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发表时间：
2012-11
影响因子：
3.4
通讯作者：
Appukuttan, Binoy
中科院分区：
文献类型：
--
作者：
Smith, Justine R.;Chipps, Timothy J.;Bias, Hoda;Pan, Yuzhen;Appukuttan, Binoy
活化白细胞粘附分子（ALCAM; CD 166）是一种免疫球蛋白超家族成员，已在几种非眼部内皮细胞群体中描述，但与眼内内皮无关。眼外系统的研究表明，ALCAM参与了视网膜血管疾病的关键过程--血管生成和白细胞跨内皮迁移。我们研究了ALCAM在人视网膜内皮细胞中的表达，并研究了已建立的血管生成和炎症刺激对表达的调节。通过RT-PCR（n = 4个供体）和Western印迹（n = 4个供体）检测从人尸体分离的原代视网膜内皮培养物中ALCAM的表达，并且通过免疫组织化学（n = 3个供体）检测完整人视网膜中ALCAM的表达。在通过RT-PCR研究的4个供体中，还检测到编码截短的可溶性同种型sALCAM的转录物。实时荧光定量RT-PCR显示ALCAM和sALCAM在主细胞因子肿瘤坏死因子（TNF）-α刺激下显著上调。然而，一般炎症刺激，脂多糖（LPS），和原型Th 1，Th 2和Th 17细胞因子，干扰素（IFN）-γ，白细胞介素（IL）-4和IL-17 A，分别不影响ALCAM或sALCAM的表达。与此相反，血管内皮生长因子（VEGF）165显著上调ALCAM的表达。在VEGF和TNF-α的存在下上调，而不是LPS、IFN-γ、IL-4和IL-17 A，表明ALCAM在某些情况下在人视网膜血管生成中的潜在作用。
Activated leukocyte cell adhesion molecule (ALCAM; CD166) is an immunoglobulin superfamily member that has been described in several non-ocular endothelial populations, but not in relation to endothelium within the eye. Studies in extraocular systems have implicated ALCAM in angiogenesis and leukocyte transendothelial migration, which are key processes in retinal vascular diseases. We investigated the expression of ALCAM in human retinal endothelium, and studied the regulation of expression by established angiogenic and inflammatory stimuli. Retinal endothelial expression of ALCAM was detected in primary retinal endothelial cultures isolated from human cadavers by RT-PCR (n = 4 donors) and Western blot (n = 4 donors), and in intact human retina by immunohistochemistry (n = 3 donors). In the 4 donors studied by RT-PCR, transcript encoding the truncated soluble isoform, sALCAM, was also detected. Quantitative real-time RT-PCR demonstrated significant up-regulation of ALCAM and sALCAM in response to stimulation with master cytokine, tumor necrosis factor (TNF)-α. However, general inflammatory stimulus, lipopolysaccharide (LPS), and the prototype Th1, Th2 and Th17 cytokines, interferon (IFN)-γ, interleukin (IL)-4 and IL-17A, respectively, did not impact ALCAM or sALCAM expression. In contrast, expression of ALCAM was significantly up-regulated by vascular endothelial growth factor (VEGF)165. Up-regulation in the presence of VEGF and TNF-α, but not LPS, IFN-γ, IL-4 and IL-17A, suggests a potential role for ALCAM in human retinal angiogenesis in some settings.
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影响因子：
9
作者：
Crane, Isabel J.;Liversidge, Janet
通讯作者：
Liversidge, Janet
DOI：
10.4049/jimmunol.1100646
发表时间：
2011-05-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Long EO
通讯作者：
Long EO
影响因子：
15.3
作者：
Arai, F;Ohneda, O;Suda, T
通讯作者：
Suda, T
DOI：
10.1073/pnas.1434429100
发表时间：
2003-09-16
影响因子：
11.1
作者：
Chi, JT;Chang, HY;Brown, PO
通讯作者：
Brown, PO
影响因子：
4.4
作者：
Chen, WQ;Esselman, WJ;Busik, JV
通讯作者：
Busik, JV