Metabolomic Expression of Laryngeal and Hindlimb Muscles in Adult versus Senescent Rats.
Metabolomic Expression of Laryngeal and Hindlimb Muscles in Adult versus Senescent Rats.
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DOI：
10.1177/00034894211014692
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发表时间：
2022-03
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影响因子：
--
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中科院分区：
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(1)确定在大鼠模型中获得喉肌的全局无偏代谢组学特征的可行性;（2）评价生物老化对喉代谢组学的影响;（3）表征老化和非老化喉肌和后肢肌之间的生化表达差异。从5只年轻成年（9月龄）和5只老年成年（32月龄）F344 BN大鼠中收获甲杓状喉肌和跖肌后肢肌肉。使用LC-MS方法处理和分析组织。将检测到的代谢物与广泛使用的代谢物数据库进行比较，并对重要代谢物进行KEGG途径富集。代谢物表达的最大差异在喉和肢体肌肉之间，在年轻组的喉和肢体之间发现了126种不同的代谢物，在老年组中发现了149种不同的代谢物。肌肉群之间的显着撞击凸显了这些组织之间的氨基酸差异。与喉肌相比，肢体肌的年龄差异更大。氨基酸代谢是四肢肌肉和喉部肌肉的关键区别。由于两个肌肉群之间差异表达的代谢产物的数量，在将骨骼肢体肌肉生理学和生物学概念应用于老年和非老年肌肉骨骼系统中的发声肌时应谨慎。年龄对喉肌的影响不如四肢肌，其机制尚待阐明。
(1) Determine the feasibility of obtaining a global, unbiased metabolomic profile on laryngeal muscle in a rat model; (2) evaluate the impact of biological aging on the laryngeal metabolome; and (3) characterize biochemical expression differences between aged and non-aged laryngeal and hindlimb muscle. Thyroarytenoid laryngeal muscle and plantaris hindlimb muscle were harvested from 5 young adult (9 months old) and 5 older adult (32 months old) F344BN rats. Tissue was processed and analyzed using LC-MS methods. Detected metabolites were compared to widely used metabolite databases and KEGG pathway enrichment was performed on significant metabolites. The greatest differences in metabolite expression were between laryngeal and limb muscle with 126 different metabolites found between laryngeal and limb within the young group and 149 different metabolites within the old group. Significant hits between muscle groups highlighted amino acid differences between these tissues. There were more robust differences with age in limb muscle compared to laryngeal muscle. Amino acid metabolism is a key difference between muscles of the limbs and larynx. Due to the number of differentially expressed metabolites between the 2 muscle groups, caution should be exercised when applying skeletal limb muscle physiology and biology concepts to the vocal muscles in both aged and non-aged musculoskeletal systems. Mechanisms underlying less robust effects of age on laryngeal muscle compared to limb muscle require elucidation.
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影响因子：
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作者：
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通讯作者：
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DOI：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1093/gerona/glt044
发表时间：
2013-12-01
影响因子：
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作者：
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通讯作者：
Connor, Nadine P.