Patterning human neuronal networks on photolithographically engineered silicon dioxide substrates functionalized with glial analogues.
Patterning human neuronal networks on photolithographically engineered silicon dioxide substrates functionalized with glial analogues.
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DOI：
10.1002/jbm.a.34813
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发表时间：
2014-05
影响因子：
4.9
通讯作者：
Shipston, Mike J.
中科院分区：
文献类型：
--
作者：
Hughes, Mark A.;Brennan, Paul M.;Bunting, Andrew S.;Cameron, Katherine;Murray, Alan F.;Shipston, Mike J.
将神经元与硅半导体连接是一项挑战，正在通过各种生物工程方法加以解决。这样的结构为我们理解神经元编码和学习提供了信息，并最终引导我们创造智能神经假体。一个基本的先决条件是决定神经元细胞的空间组织。我们试图使用胎牛血清激活的聚对二甲苯-C的电子照相定义的阵列来图案化神经元。我们使用纯化的人神经元细胞系[隆德人中脑（LUHMES）]来确定神经元在芯片上分离时是否保持活力，或者它们是否需要支持细胞基质。当分离培养时，LUHMES神经元未能形成图案，并且没有显示出任何分化的形态学迹象。因此，我们寻求一种细胞类型，其中prepatterning聚对二甲苯地区，假设这种细胞模板将使继发性神经元粘附和网络形成。从一系列测试的细胞系中，人胚肾（HEK）293细胞以最高的准确度进行了图案化。LUHMES神经元粘附于预先建立的HEK 293细胞簇，并且这种共培养环境促进神经元的形态分化。神经突在贴壁细胞胞体岛之间延伸，形成正交排列的神经元网络。HEK 293细胞似乎履行类似于神经胶质的作用，决定细胞粘附，并产生有利于神经元存活的环境。我们接下来用生长较慢的神经胶质瘤衍生的前体细胞替换HEK 293细胞。这些原代人类细胞在聚对二甲苯上精确地形成图案，并为神经元粘附提供了类似的有效支架。这些发现推进了这种微制造兼容平台在神经元图案化中的应用。© 2013作者。生物医学材料研究杂志，由威利期刊公司出版。J Biomed Mater Res Part A：102A：1350-1360，2014.
Interfacing neurons with silicon semiconductors is a challenge being tackled through various bioengineering approaches. Such constructs inform our understanding of neuronal coding and learning and ultimately guide us toward creating intelligent neuroprostheses. A fundamental prerequisite is to dictate the spatial organization of neuronal cells. We sought to pattern neurons using photolithographically defined arrays of polymer parylene-C, activated with fetal calf serum. We used a purified human neuronal cell line [Lund human mesencephalic (LUHMES)] to establish whether neurons remain viable when isolated on-chip or whether they require a supporting cell substrate. When cultured in isolation, LUHMES neurons failed to pattern and did not show any morphological signs of differentiation. We therefore sought a cell type with which to prepattern parylene regions, hypothesizing that this cellular template would enable secondary neuronal adhesion and network formation. From a range of cell lines tested, human embryonal kidney (HEK) 293 cells patterned with highest accuracy. LUHMES neurons adhered to pre-established HEK 293 cell clusters and this coculture environment promoted morphological differentiation of neurons. Neurites extended between islands of adherent cell somata, creating an orthogonally arranged neuronal network. HEK 293 cells appear to fulfill a role analogous to glia, dictating cell adhesion, and generating an environment conducive to neuronal survival. We next replaced HEK 293 cells with slower growing glioma-derived precursors. These primary human cells patterned accurately on parylene and provided a similarly effective scaffold for neuronal adhesion. These findings advance the use of this microfabrication-compatible platform for neuronal patterning. © 2013 The Authors. Journal ofBiomedicalMaterials Research Part APublished byWiley Periodicals, Inc.Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1350–1360, 2014.
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影响因子：
3.8
作者：
Chang, Chia-Hao;Yu, Feng-Yih;Liu, Biing-Hui
通讯作者：
Liu, Biing-Hui
影响因子：
23.9
作者：
Pollard, Steven M.;Yoshikawa, Koichi;Dirks, Peter
通讯作者：
Dirks, Peter
长期神经胶质和神经元培养的粘附和生长在戊烯-C上。
影响因子：
3.7
作者：
Delivopoulos E;Murray AF
通讯作者：
Murray AF
影响因子：
3.8
作者：
GRAHAM, FL;SMILEY, J;NAIRN, R
通讯作者：
NAIRN, R
影响因子：
4.6
作者：
GROSS, GW
通讯作者：
GROSS, GW