The AAA+ ATPases and HflB/FtsH proteases of 'Candidatus Phytoplasma mali': phylogenetic diversity, membrane topology, and relationship to strain virulence.
The AAA+ ATPases and HflB/FtsH proteases of 'Candidatus Phytoplasma mali': phylogenetic diversity, membrane topology, and relationship to strain virulence.
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“Candidatus mali”的 AAA ATP 酶和 HflB/FtsH 蛋白酶：系统发育多样性、膜拓扑结构以及与菌株毒力的关系
DOI：
10.1094/mpmi-09-12-0221-r
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Schneider B
中科院分区：
文献类型：
--
作者：
Seemüller E;Sule S;Kube M;Jelkmann W;Schneider B
先前的研究揭示了‘苹果植原体’菌株的植物致病性数据与ATP 00464型hflB基因片段的DNA序列变异性的相关性。为了进一步研究这种关系，从许多代表性的轻度、中度和重度菌株中对先前在“苹果酸钙”菌株AT的基因组中注释的所有不同基因进行完全测序和分析。重新注释表明序列编码六种AAA+ ATP酶和六种HflB蛋白酶。每九个不同的推导AAA+蛋白，检查形成一个连贯的系统发育簇。然而，在这些组中，三个ATP酶和三个蛋白酶的序列从轻度和严重的菌株聚类遥远，根据他们的毒力。这种分组得到了与毒性相关的氨基酸取代的关联的支持。另一个发现是ATP酶AP 11的全长基因只能在轻度和中度菌株中鉴定。膜拓扑结构的预测表明，约一半的AAA+蛋白质的长的ATP酶和蛋白酶携带的C-末端尾部是细胞外的，pueridine面对的环境中的筛管。因此，它们可能参与病原体-宿主相互作用，并可能损害韧皮部功能，这是植原体感染的主要影响。所有的全长基因检查出现转录活性和所有推导的肽显示的关键位置指示蛋白质功能。
Previous examination revealed a correlation of phytopathogenic data of ‘CandidatusPhytoplasma mali’ strains and the DNA sequence variability of a type ATP00464hflBgene fragment. To further investigate such a relationship, all distinct genes previously annotated ashflBin the genome of ‘Ca.P. mali’ strain AT were fully sequenced and analyzed from a number of representative mild, moderate, and severe strains. The re-annotation indicated that the sequences encode six AAA+ ATPases and six HflB proteases. Each of the nine distinct deduced AAA+ proteins that were examined formed a coherent phylogenetic cluster. However, within these groups, sequences of three ATPases and three proteases from mild and severe strains clustered distantly, according to their virulence. This grouping was supported by an association with virulence-related amino acid substitutions. Another finding was that full-length genes from ATPase AP11 could only be identified in mild and moderate strains. Prediction of the membrane topology indicated that the long ATPase- and protease-carrying C-terminal tails of approximately half of the AAA+ proteins are extracellular, putatively facing the environment of the sieve tubes. Thus, they may be involved in pathogen–host interactions and may compromise phloem function, a major effect of phytoplasma infection. All full-length genes examined appear transcriptionally active and all deduced peptides show the key positions indicative for protein function.
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影响因子：
1.8
作者：
M. Rott;W. Jelkmann
通讯作者：
M. Rott;W. Jelkmann
影响因子：
30.8
作者：
Oshima, K;Kakizawa, S;Namba, S
通讯作者：
Namba, S
影响因子：
10.7
作者：
Dagan, T;Talmor, Y;Graur, D
通讯作者：
Graur, D
基于 HflB 基因的“Candidatus phytoplasma mali”菌株的植物病原分类以及菌株组成决定多重感染苹果树毒力的证据。
DOI：
10.1094/mpmi-05-11-0126
发表时间：
2011
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
E. Seemüller;M. Kampmann;Emese Kiss;B. Schneider
通讯作者：
B. Schneider
影响因子：
4.4
作者：
Kube M;Schneider B;Kuhl H;Dandekar T;Heitmann K;Migdoll AM;Reinhardt R;Seemüller E
通讯作者：
Seemüller E