2D-DIGE proteome analysis on the platelet proteins of patients with major depression.
2D-DIGE proteome analysis on the platelet proteins of patients with major depression.
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DOI：
10.1186/1477-5956-12-1
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发表时间：
2014-01-03
期刊：
影响因子：
2
通讯作者：
Chen TY
中科院分区：
文献类型：
--
作者：
Huang TL;Sung ML;Chen TY
血小板活化与重度抑郁症的精神病理有关。我们试图从重度抑郁症患者的血小板中寻找和鉴定蛋白质生物标志物。应用高分辨率二维差分凝胶电泳（2D-DIGE）、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）、Western blot和生物信息学工具检测了10例重度抑郁症患者和10例健康对照者的血小板蛋白。重度抑郁症急性期患者与健康对照者8种蛋白水平差异有统计学意义。重度抑郁症患者的蛋白二硫异构酶A3 （PDIA3）和f -肌动蛋白封盖蛋白亚基β (CAPZB)水平高于健康对照组。重度抑郁症患者纤维蛋白原β链（FIBB）、纤维蛋白原γ链（FIBG）、视黄酸受体β (RARB)、谷胱甘肽过氧化物酶1 （GPX1）、SH3结构域含蛋白19 （SH319）和t复合物蛋白1亚基β (TCPB)水平低于健康对照组。血小板提供了与重度抑郁症相关的炎症/免疫、氧化应激和神经发生的途径和过程的宝贵信息。
Platelet activation is related to the psychopathology of major depression. We attempted to search and identify protein biomarkers from the platelets of patients with major depression. High resolution two-dimensional Differential Gel Electrophoresis (2D-DIGE), the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), Western blot, and bioinformatic tools were applied to examine the platelet proteins of 10 patients with major depression and 10 healthy controls. The levels of 8 proteins were significantly different between the patients with major depression in the acute phase and healthy controls. The levels of protein disulfide-isomerase A3 (PDIA3) and F-actin-capping protein subunit beta (CAPZB) were higher in patients with major depression than in healthy controls. The levels of fibrinogen beta chain (FIBB), fibrinogen gamma chain (FIBG), retinoic acid receptor beta (RARB), glutathione peroxidase 1 (GPX1), SH3 domain-containing protein 19 (SH319), and T-complex protein 1 subunit beta (TCPB) were lower in patients with major depression than in healthy controls. Platelet provided valuable information about the pathways and processes of inflammation/immunity, oxidative stress, and neurogenesis, related to major depression.
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影响因子：
10.6
作者：
Karege, F;Bondolfi, G;Bertschy, G
通讯作者：
Bertschy, G
DOI：
10.1073/pnas.0406788101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Evans, SJ;Choudary, PV;Akil, H
通讯作者：
Akil, H
影响因子：
11
作者：
Kim, YK;Suh, IB;Licinio, J
通讯作者：
Licinio, J
影响因子：
10.6
作者：
Dowlati, Yekta;Herrmann, Nathan;Lanctot, Krista L.
通讯作者：
Lanctot, Krista L.
影响因子：
1.4
作者：
Angerio, Allan D.
通讯作者：
Angerio, Allan D.