Cellular analysis of cleavage-stage chick embryos reveals hidden conservation in vertebrate early development.
Cellular analysis of cleavage-stage chick embryos reveals hidden conservation in vertebrate early development.
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DOI：
10.1242/dev.118604
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发表时间：
2015-04-01
期刊：
影响因子：
--
通讯作者：
Sheng G
中科院分区：
文献类型：
--
作者：
Nagai H;Sezaki M;Kakiguchi K;Nakaya Y;Lee HC;Ladher R;Sasanami T;Han JY;Yonemura S;Sheng G
鸟类和哺乳动物，具有相似的原肠胚后发育的遗传学上接近的哺乳动物，在受精后卵裂模式中表现出很少的保守性。来自小鼠的数据表明，在卵裂阶段的细胞形态发生和分子信号传导在后期（囊胚和原肠胚）阶段的谱系特化中起重要作用。然而，由于鸡胚不易获得，人们对卵裂期鸡胚知之甚少。鸡的这一发育时期发生在产蛋之前，包括几个鸟类胚胎学的基本过程，包括合子基因激活（ZGA）和胚盘细胞层的增加。我们进行了形态和细胞分析的卵裂期鸡胚覆盖前产卵发展的前半部分，从Eyal-Giladi和Kochav阶段（EGK-）I EGK-V。扫描电子显微镜显示显着的亚细胞细节的卵裂球细胞化和亚胚腔形成。磷酸化RNA聚合酶II免疫染色表明，ZGA在小鸡开始在早期EGK-III在第7至第8核分裂周期，与其他富含蛋黄的脊椎动物（如斑马鱼和非洲爪蟾）报告的时间。EGK-III后细胞层数的增加不是定向细胞分裂的直接结果。最后，我们提出的证据表明，在斑马鱼胚胎中，卵黄合胞体层形成后，EGK-V的鸟类胚胎。我们的数据表明，鸟类卵裂阶段的发展的几个基本特征类似于那些在富含蛋黄的海洋动物物种，揭示保护脊椎动物早期发育。这种保守性是否有助于形态发生学上的支持，或反映了趋同进化中的发育可塑性。总结：早期鸡胚具有以前不被重视的特征，如合子基因激活和卵黄合胞体层形成的时间。
Birds and mammals, phylogenetically close amniotes with similar post-gastrula development, exhibit little conservation in their post-fertilization cleavage patterns. Data from the mouse suggest that cellular morphogenesis and molecular signaling at the cleavage stage play important roles in lineage specification at later (blastula and gastrula) stages. Very little is known, however, about cleavage-stage chick embryos, owing to their poor accessibility. This period of chick development takes place before egg-laying and encompasses several fundamental processes of avian embryology, including zygotic gene activation (ZGA) and blastoderm cell-layer increase. We have carried out morphological and cellular analyses of cleavage-stage chick embryos covering the first half of pre-ovipositional development, from Eyal-Giladi and Kochav stage (EGK-) I to EGK-V. Scanning electron microscopy revealed remarkable subcellular details of blastomere cellularization and subgerminal cavity formation. Phosphorylated RNA polymerase II immunostaining showed that ZGA in the chick starts at early EGK-III during the 7th to 8th nuclear division cycle, comparable with the time reported for other yolk-rich vertebrates (e.g. zebrafish and Xenopus). The increase in the number of cell layers after EGK-III is not a direct consequence of oriented cell division. Finally, we present evidence that, as in the zebrafish embryo, a yolk syncytial layer is formed in the avian embryo after EGK-V. Our data suggest that several fundamental features of cleavage-stage development in birds resemble those in yolk-rich anamniote species, revealing conservation in vertebrate early development. Whether this conservation lends morphogenetic support to the anamniote-to-amniote transition in evolution or reflects developmental plasticity in convergent evolution awaits further investigation. Summary: Early chick embryos share previously unappreciated features with anamniote embryos such as the timing of zygotic gene activation and yolk syncytial layer formation.
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影响因子：
56.9
作者：
Leichsenring, Manuel;Maes, Julia;Onichtchouk, Daria
通讯作者：
Onichtchouk, Daria
DOI：
10.1016/s0022-5320(74)90049-5
发表时间：
1974-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
GIPSON, I
通讯作者：
GIPSON, I
影响因子：
3.7
作者：
Lee HC;Choi HJ;Park TS;Lee SI;Kim YM;Rengaraj D;Nagai H;Sheng G;Lim JM;Han JY
通讯作者：
Han JY
影响因子：
4.5
作者：
Mathavan S;Lee SG;Mak A;Miller LD;Murthy KR;Govindarajan KR;Tong Y;Wu YL;Lam SH;Yang H;Ruan Y;Korzh V;Gong Z;Liu ET;Lufkin T
通讯作者：
Lufkin T
影响因子：
2.7
作者：
KOCHAV, S;GINSBURG, M;EYALGILADI, H
通讯作者：
EYALGILADI, H