Cleavage events and sperm dynamics in chick intrauterine embryos.
Cleavage events and sperm dynamics in chick intrauterine embryos.
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DOI：
10.1371/journal.pone.0080631
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Han JY
中科院分区：
文献类型：
--
作者：
Lee HC;Choi HJ;Park TS;Lee SI;Kim YM;Rengaraj D;Nagai H;Sheng G;Lim JM;Han JY
本研究旨在通过一种产卵前的非侵入性取卵技术来阐明鸡胚胎早期胚胎发生的详细情况。从95只54~56周龄的循环蛋鸡中取出白色来航蛋，并在明场和荧光图像下对其形态发生进行时程观察。与哺乳动物不同的是，在整个胚胎发育过程中，都观察到了不对称的卵裂来产生前胚层细胞。前两次分裂是同步进行的，十字形分裂后形成4个极化的前胚层细胞。然后，异步性分裂继续以放射状方式进行，初始卵裂区的细胞总体尺寸小于远端区域的细胞尺寸。无论合子核如何形成，均可见大量精子。浓缩精子头主要存在于卵黄周间隙和细胞质中，在卵黄区很少，而解聚精子头仅在卵黄中可见。综上所述，在鸡胚胎发育过程中，精子动力学和早期卵裂事件与哺乳动物胚胎有明显差异，表现为将多余的精子穿透到多个区域的极化分裂。
This study was undertaken to elucidate detailed event of early embryogenesis in chicken embryos using a noninvasive egg retrieval technique before oviposition. White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both bright field and fluorescent image in a time course manner. Differing from mammals, asymmetric cleavage to yield preblastodermal cells was observed throughout early embryogenesis. The first two divisions occurred synchronously and four polarized preblastodermal cells resulted after cruciform cleavage. Then, asynchronous cleavage continued in a radial manner and overall cell size in the initial cleavage region was smaller than that in the distal area. Numerous sperms were visible, regardless of zygotic nuclei formation. Condensed sperm heads were present mainly in the perivitelline space and cytoplasm, and rarely in the yolk region, while decondensed sperm heads were only visible in the yolk. In conclusion, apparent differences in sperm dynamics and early cleavage events compared with mammalian embryos were detected in chick embryo development, which demonstrated polarized cleavage with penetrating supernumerary sperm into multiple regions.
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影响因子：
2.7
作者：
EYALGILADI, H;KOCHAV, S
通讯作者：
KOCHAV, S
影响因子：
2.5
作者：
Park, HJ;Park, TS;Han, JY
通讯作者：
Han, JY
影响因子：
4.4
作者：
BRAMWELL, RK;MARKS, HL;HOWARTH, B
通讯作者：
HOWARTH, B
DOI：
10.1530/jrf.0.1010353
发表时间：
1994-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BIRKHEAD, TR;SHELDON, BC;FLETCHER, F
通讯作者：
FLETCHER, F
影响因子：
1.5
作者：
Olsen, MW
通讯作者：
Olsen, MW