Triplex-forming oligonucleotides: a third strand for DNA nanotechnology.
Triplex-forming oligonucleotides: a third strand for DNA nanotechnology.
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DOI：
10.1093/nar/gkx1230
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发表时间：
2018-02-16
影响因子：
14.9
通讯作者：
Rusling DA
中科院分区：
文献类型：
--
作者：
Chandrasekaran AR;Rusling DA
DNA自组装已被证明是一种有用的自下而上的策略，用于构建用户定义的纳米级物体，晶格和设备。这些结构的设计在很大程度上依赖于利用简单的碱基配对规则和作为二级结构元件的双螺旋结构域的形成。然而，涉及特定非规范碱基-碱基相互作用的其他螺旋形式已经将新的范例引入DNA工程化过程。其中最值得注意的是通过双链体大沟内的第三链的结合产生的三链复合物，产生三螺旋（“三链体”）结构。将三链体与其双链体对应物区分开的序列、结构和组装要求允许设计用于动态和/或结构目的的纳米结构，以及将非核酸组分靶向纳米结构支架内的精确位置的手段。在这里，我们回顾了三链体的性质，已被证明是有用的DNA纳米结构的工程，重点是应用程序，迄今为止还没有可能的双链体形成单独的。
DNA self-assembly has proved to be a useful bottom-up strategy for the construction of user-defined nanoscale objects, lattices and devices. The design of these structures has largely relied on exploiting simple base pairing rules and the formation of double-helical domains as secondary structural elements. However, other helical forms involving specific non-canonical base-base interactions have introduced a novel paradigm into the process of engineering with DNA. The most notable of these is a three-stranded complex generated by the binding of a third strand within the duplex major groove, generating a triple-helical (‘triplex’) structure. The sequence, structural and assembly requirements that differentiate triplexes from their duplex counterparts has allowed the design of nanostructures for both dynamic and/or structural purposes, as well as a means to target non-nucleic acid components to precise locations within a nanostructure scaffold. Here, we review the properties of triplexes that have proved useful in the engineering of DNA nanostructures, with an emphasis on applications that hitherto have not been possible by duplex formation alone.
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影响因子：
14.9
作者：
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通讯作者：
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