Transcriptome and metabolome analyses reveal the responses of brown planthoppers to RH resistant rice cultivar.
Transcriptome and metabolome analyses reveal the responses of brown planthoppers to RH resistant rice cultivar.
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DOI：
10.3389/fphys.2022.1018470
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
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褐飞虱（Nilaparvata lugens（Stål））为半翅目（Hemiptera）飞虱科（Delphacidae）飞虱属（Delphacidae）飞虱属（Nilaparvata lugens）害虫，是亚洲水稻生产中危害最严重的害虫之一。应用抗虫水稻品种是目前防治褐飞虱的主要手段之一。了解褐飞虱取食抗虫水稻的生理反应机制是保持水稻产量的关键。在这里，我们测量的生态适合度和分析的整体转录组和代谢组的BPH饲养的敏感品种台中本地1（TN1）和抗性品种Rathu Heenati（RH）。结果表明，RH显著降低褐飞虱的存活率、雌成虫体重、蜜露分泌量、产卵量和脂肪含量。我们确定了333个上调和486个下调的基因在BPH饲养RH。这些基因主要参与能量代谢、氨基酸代谢、激素合成和维生素代谢途径。我们还检测到145差异积累的代谢产物在BPH饲养RH植物相比，BPH饲养TN1植物，包括多种碳水化合物，氨基酸，脂质和一些核苷。转录组和代谢组的综合分析表明，五个途径，包括淀粉，蔗糖和半乳糖代谢，改变。这些途径的网络随后被可视化。我们的研究结果提供了深入了解的代谢物积累的机制，在褐飞虱饲养的RH水稻品种。研究结果有助于我们更好地了解抗虫水稻品种如何对抗褐飞虱的侵扰，这对褐飞虱的综合治理具有重要意义。
The brown planthopper (BPH) Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) is one of the most destructive rice pests in Asia. The application of insect-resistant rice cultivars is currently one of the principal means of controlling BPH. Understanding the physiological response mechanisms of BPH feeding on insect-resistant rice is the key for maintaining rice yield. Here, we measured the ecological fitness and analyzed the whole-body transcriptome and metabolome of BPH reared on susceptible cultivar Taichung Native 1 (TN1) and resistant cultivar Rathu Heenati (RH). Our results showed that RH significantly decreased the survival rate, female adult weight, honeydew secretion, the number of eggs laid per female and fat content of BPH. We identified 333 upregulated and 486 downregulated genes in BPH feeding on RH. These genes were mainly involved in energy metabolism, amino acid metabolism, hormone synthesis and vitamin metabolism pathways. We also detected 145 differentially accumulated metabolites in BPH reared on RH plants compared to BPH reared on TN1 plants, including multiple carbohydrates, amino acids, lipids, and some nucleosides. Combined analyses of transcriptome and metabolome showed that five pathways, including starch, sucrose, and galactose metabolism, were altered. The network for these pathways was subsequently visualized. Our results provide insights into the mechanisms of metabolite accumulation in BPH feeding on the RH rice variety. The results could help us better understand how insect-resistant rice cultivars combat BPH infestation, which is important for the comprehensive management of BPH.
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影响因子：
4.4
作者：
Srivastava V;Obudulu O;Bygdell J;Löfstedt T;Rydén P;Nilsson R;Ahnlund M;Johansson A;Jonsson P;Freyhult E;Qvarnström J;Karlsson J;Melzer M;Moritz T;Trygg J;Hvidsten TR;Wingsle G
通讯作者：
Wingsle G
影响因子：
3.8
作者：
Andersen, Svend Olav
通讯作者：
Andersen, Svend Olav
影响因子：
4.1
作者：
Gorman, Kevin;Liu, Zewen;Nauen, Ralf
通讯作者：
Nauen, Ralf
影响因子：
7.4
作者：
Hao, Peiying;Liu, Caixiang;He, Guangcun
通讯作者：
He, Guangcun
影响因子：
4.1
作者：
Hu, Jie;Cheng, Mingxing;He, Yuqing
通讯作者：
He, Yuqing