T-cell recognition of prostatic peptides in men with chronic prostatitis/chronic pelvic pain syndrome.
T-cell recognition of prostatic peptides in men with chronic prostatitis/chronic pelvic pain syndrome.
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DOI：
10.1016/j.juro.2009.07.067
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发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Alexander RB
中科院分区：
文献类型：
--
作者：
Kouiavskaia DV;Southwood S;Berard CA;Klyushnenkova EN;Alexander RB
One of the potential etiologies for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is autoimmunity. We wished to determine if T cells from a group of men with CP/CPPS would recognize peptides derived from the normal self prostatic proteins prostate specific antigen (PSA) and prostatic acid phosphatase (PAP). CD4 T cells purified from the peripheral blood of 31 patients with CP/CPPS and from the buffy coat preparation of 27 normal male blood donors were stimulated in vitro with a panel of immunogenic peptides from PSA and PAP and assayed for reactivity with the peptides by IFN-γ ELISPOT assay. Intermediate resolution HLA typing was performed by PCR. Peptides were also tested in binding assay against different class II alleles. Peptide PAP173-192 was recognized more frequently by CD4 T cells from the patients with CP/CPPS compared to the healthy donors. Recognition of PSA peptides was not statistically different comparing cases to normal male blood donors individually. Peptide reactivity was more commonly observed in cases compared to normal male blood donors for any PSA peptide or any tested peptide. All peptides showed a high level of promiscuity in the binding assays. There was no association of cases with any specific HLA class II phenotype at intermediate resolution. CD4 T cells from patients with CP/CPPS have a higher frequency of recognition of the self prostatic proteins PAP and PSA compared to normal male blood donors. The data provide further evidence in support of the role of autoimmunity in some men with CP/CPPS.
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影响因子：
3.9
作者：
Klyushnenkova, EN;Ponniah, S;Alexander, RB
通讯作者：
Alexander, RB
影响因子：
8.6
作者：
Motrich, RD;Maccioni, M;Rivero, VE
通讯作者：
Rivero, VE
影响因子：
11.5
作者：
Klyushnenkova, EN;Link, J;Alexander, RB
通讯作者：
Alexander, RB
影响因子：
2.1
作者：
Alexander, RB;Brady, F;Ponniah, S
通讯作者：
Ponniah, S
影响因子：
--
作者：
Sidney, J;Southwood, S;Grey, H M
通讯作者：
Grey, H M