SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data.
SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data.
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DOI：
10.1186/1471-2105-11-485
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发表时间：
2010-09-27
影响因子：
3
通讯作者：
Biggs PJ
中科院分区：
文献类型：
--
作者：
Cox MP;Peterson DA;Biggs PJ
Illumina的第二代测序平台在现代DNA和RNA测序工作中发挥着越来越重要的作用。然而，目前缺乏快速、简单、标准化和独立的运行质量测量，以及基于读取级质量数据处理用于下游应用的序列的工具。我们提出SolexaQA，一个用户友好的软件包，旨在生成详细的统计数据和一目了然的序列数据质量的图形快速和自动化的方式。该软件包包含相关软件，可使用单个读段内碱基的质量评分动态修剪序列。SolexaQA软件包可在几分钟内生成标准化输出，从而便于在流动池通道和机器运行之间进行快速比较，并提供即时诊断信息，以指导下游分析的序列数据操作。
Illumina's second-generation sequencing platform is playing an increasingly prominent role in modern DNA and RNA sequencing efforts. However, rapid, simple, standardized and independent measures of run quality are currently lacking, as are tools to process sequences for use in downstream applications based on read-level quality data. We present SolexaQA, a user-friendly software package designed to generate detailed statistics and at-a-glance graphics of sequence data quality both quickly and in an automated fashion. This package contains associated software to trim sequences dynamically using the quality scores of bases within individual reads. The SolexaQA package produces standardized outputs within minutes, thus facilitating ready comparison between flow cell lanes and machine runs, as well as providing immediate diagnostic information to guide the manipulation of sequence data for downstream analyses.
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