Yu Ping Feng San, an ancient Chinese herbal decoction containing Astragali Radix, Atractylodis Macrocephalae Rhizoma and Saposhnikoviae Radix, regulates the release of cytokines in murine macrophages.
Yu Ping Feng San, an ancient Chinese herbal decoction containing Astragali Radix, Atractylodis Macrocephalae Rhizoma and Saposhnikoviae Radix, regulates the release of cytokines in murine macrophages.
复制标题
DOI：
10.1371/journal.pone.0078622
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tsim KW
中科院分区：
文献类型：
--
作者：
Du CY;Choi RC;Zheng KY;Dong TT;Lau DT;Tsim KW
郁平风散（YPFS）是一种中药汤剂，由黄芪、白术、防风三种药材按1∶2∶1的质量比配制而成。临床上，YPFS已被广泛用于调节免疫功能；但其作用机制尚不清楚。在这里，我们通过详细分析YPFS的化学和生物学特性来解决这个问题。采用快速分辨液相色谱-质谱联用技术，测定了不同药材中15种化学成分的含量，并作为药材提取物标准化的对照物。一般来说，与单药或双药组合物相比，YPFS制剂中所选择的化学标记物的数量更高。为了揭示YPFS的免疫功能，我们将标准提取物应用于培养的小鼠巨噬细胞。YPFS治疗通过增强i -κB α降解，激活NF-κB，刺激促炎细胞因子mRNA和蛋白表达。相反，在脂多糖（LPS）诱导的慢性炎症模型中，应用YPFS可显著抑制促炎细胞因子的表达。此外，YPFS还能上调培养巨噬细胞的吞噬活性。因此，这些结果支持了YPFS在调节巨噬细胞细胞因子释放中的双向免疫调节作用。
Yu Ping Feng San (YPFS), a Chinese herbal decoction, is composed of Astragali Radix (AR; Huangqi), Atractylodis Macrocephalae Rhizoma (AMR; Baizhu) and Saposhnikoviae Radix (SR; Fangfeng) in a weight ratio of 1∶2∶1. Clinically, YPFS has been widely used to regulate immune functions; however, the action mechanism of it is not known. Here, we addressed this issue by providing detail analyses of chemical and biological properties of YPFS. By using rapid resolution liquid chromatography coupled with mass spectrometry, fifteen chemicals deriving from different herbs of YPFS were determined, and which served as a control for the standardization of the herbal extract of YPFS. In general, the amounts of chosen chemical markers were higher in a preparation of YPFS as compared to that of single herb or two-herb compositions. In order to reveal the immune functions of YPFS, the standardized extract was applied onto cultured murine macrophages. The treatment of YPFS stimulated the mRNA and protein expressions of pro-inflammatory cytokines via activation of NF-κB by enhancing IκBα degradation. In contrast, the application of YPFS suppressed the expressions of pro-inflammatory cytokines significantly in the lipopolysaccharide (LPS)-induced chronic inflammation model. In addition, YPFS could up regulate the phagocytic activity in cultured macrophages. These results therefore supported the bi-directional immune-modulatory roles of YPFS in regulating the releases of cytokines from macrophages.
登录
查看更多内容
影响因子：
5.7
作者：
Poon, PMK;Wong, CK;Lam, CWK
通讯作者：
Lam, CWK
影响因子：
2.7
作者：
Gao, Qiu T.;Cheung, Jerry K. H.;Tsim, Karl W. K.
通讯作者：
Tsim, Karl W. K.
影响因子：
7.2
作者：
Chiu, Shih-Che;Lai, Yuen-Liang;Chen, Yu-Jen
通讯作者：
Chen, Yu-Jen
影响因子：
4.9
作者：
Gao Q;Li J;Cheung JK;Duan J;Ding A;Cheung AW;Zhao K;Li WZ;Dong TT;Tsim KW
通讯作者：
Tsim KW
影响因子：
5.1
作者：
Guo, Weixiao;Sun, Jingjing;Feng, Haihua
通讯作者：
Feng, Haihua