A single-cell analysis of thymopoiesis and thymic iNKT cell development in pigs.
A single-cell analysis of thymopoiesis and thymic iNKT cell development in pigs.
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DOI：
10.1016/j.celrep.2022.111050
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发表时间：
2022-07-05
期刊：
影响因子：
8.8
通讯作者：
Driver, John P.
中科院分区：
文献类型：
--
作者：
Gu, Weihong;Madrid, Darling Melany C.;Joyce, Sebastian;Driver, John P.
猪免疫系统的许多方面仍然知之甚少，这对改善猪的健康和利用猪作为临床前模型构成了障碍。在这里，我们采用单细胞 RNA 测序 (scRNA-seq) 来创建青春期早期猪胸腺的细胞图谱。我们的数据显示了与人类胸腺细胞相比的保守特征以及细胞状态和细胞类型的物种特异性差异。我们还描述了几种非常规 T 细胞类型，其基因表达谱与先天效应功能相关。这包括对超过 11,000 个分化不变自然杀伤 T (iNKT) 细胞的细胞普查，结果表明猪 iNKT 细胞的功能多样性与在小鼠中建立的 iNKT0/1/2/17 子集分化范例有很大不同。我们的数据描述了猪胸腺生成和 iNKT 细胞成熟中的关键分化事件，并为猪 T 细胞发育提供了重要见解。顾等人。使用单细胞 RNA 测序来表征猪的胸腺生成。这揭示了与了解猪细胞免疫相关的亚群和调控网络。他们的分析包括对 11,000 多个胸腺 iNKT 细胞的普查，结果表明猪 iNKT 细胞分化不同于在小鼠中建立的 iNKT0/1/2/17 范式。
Many aspects of the porcine immune system remain poorly characterized, which poses a barrier to improving swine health and utilizing pigs as preclinical models. Here, we employ single-cell RNA sequencing (scRNA-seq) to create a cell atlas of the early-adolescent pig thymus. Our data show conserved features as well as species-specific differences in cell states and cell types compared with human thymocytes. We also describe several unconventional T cell types with gene expression profiles associated with innate effector functions. This includes a cell census of more than 11,000 differentiating invariant natural killer T (iNKT) cells, which reveals that the functional diversity of pig iNKT cells differs substantially from the iNKT0/1/2/17 subset differentiation paradigm established in mice. Our data characterize key differentiation events in porcine thymopoiesis and iNKT cell maturation and provide important insights into pig T cell development. Gu et al. use single-cell RNA sequencing to characterize thymopoiesis in pigs. This reveals subpopulations and regulatory networks relevant for understanding cellular immunity in swine. Their analysis includes a census of more than 11,000 thymic iNKT cells, which shows that pig iNKT cell differentiation differs from the iNKT0/1/2/17 paradigm established in mice.
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