Sex and estrous cycle affect experience-dependent plasticity in mouse primary visual cortex.
Sex and estrous cycle affect experience-dependent plasticity in mouse primary visual cortex.
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DOI：
10.1371/journal.pone.0282349
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
性激素可以影响细胞生理学并调节突触可塑性，但并不总是清楚体外发现的性别依赖性差异是否或如何以大脑功能二态性的形式表达出来。从历史上看，大多数实验神经科学都是仅使用雄性动物进行的，并且文献对于将雌性小鼠纳入其中是否会由于固有的性别差异或内源性动情周期而引入变异性基本上保持沉默。尽管在美国国立卫生研究院指示性别应作为脊椎动物研究的一个因素之后，这种情况开始发生变化，但信息的缺乏引发了围绕如何设计实验对照并将现有知识应用于更多异质种群的实际问题。各种研究表明，视觉处理可能会受到性别和动情周期阶段的影响。由于这些原因，我们进行了一系列体内电生理学实验，以表征雄性和雌性小鼠初级视觉皮层 (V1) 的基线视觉功能和经验依赖性可塑性。我们发现性别和发情阶段对基线敏锐度测量没有统计学上的显着影响，但性别和发情阶段都可以调节两种机制不同形式的经验依赖性皮质可塑性。我们还证明，通过适当的标准化可以在很大程度上控制由此产生的变异性。这些发现表明，V1 可塑性可用于机制研究，重点关注性激素如何影响哺乳动物皮质中的体验依赖性可塑性。
Sex hormones can affect cellular physiology and modulate synaptic plasticity, but it is not always clear whether or how sex-dependent differences identified in vitro express themselves as functional dimorphisms in the brain. Historically, most experimental neuroscience has been conducted using only male animals and the literature is largely mute about whether including female mice in will introduce variability due to inherent sex differences or endogenous estrous cycles. Though this is beginning to change following an NIH directive that sex should be included as a factor in vertebrate research, the lack of information raises practical issues around how to design experimental controls and apply existing knowledge to more heterogeneous populations. Various lines of research suggest that visual processing can be affected by sex and estrous cycle stage. For these reasons, we performed a series of in vivo electrophysiological experiments to characterize baseline visual function and experience-dependent plasticity in the primary visual cortex (V1) of male and female mice. We find that sex and estrous stage have no statistically significant effect on baseline acuity measurements, but that both sex and estrous stage have can modulate two mechanistically distinct forms of experience dependent cortical plasticity. We also demonstrate that resulting variability can be largely controlled with appropriate normalizations. These findings suggest that V1 plasticity can be used for mechanistic studies focusing on how sex hormones effect experience dependent plasticity in the mammalian cortex.
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DOI：
10.1530/jrf.0.0270233
发表时间：
1971-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CHAMPLIN, AK
通讯作者：
CHAMPLIN, AK
影响因子：
5.3
作者：
Blume, Shannon R.;Freedberg, Mari;Rosenkranz, J. Amiel
通讯作者：
Rosenkranz, J. Amiel
影响因子：
3.3
作者：
Daniel, JM;Hulst, JL;Lee, CD
通讯作者：
Lee, CD
影响因子：
4.1
作者：
Cardoso, CC;Pereira, RTS;Abdalla, FMF
通讯作者：
Abdalla, FMF
影响因子：
8.2
作者：
Beery AK;Zucker I
通讯作者：
Zucker I