The Arabidopsis TOR Kinase Specifically Regulates the Expression of Nuclear Genes Coding for Plastidic Ribosomal Proteins and the Phosphorylation of the Cytosolic Ribosomal Protein S6.
The Arabidopsis TOR Kinase Specifically Regulates the Expression of Nuclear Genes Coding for Plastidic Ribosomal Proteins and the Phosphorylation of the Cytosolic Ribosomal Protein S6.
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DOI：
10.3389/fpls.2016.01611
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发表时间：
2016
影响因子：
5.6
通讯作者：
Meyer C
中科院分区：
文献类型：
--
作者：
Dobrenel T;Mancera-Martínez E;Forzani C;Azzopardi M;Davanture M;Moreau M;Schepetilnikov M;Chicher J;Langella O;Zivy M;Robaglia C;Ryabova LA;Hanson J;Meyer C
蛋白质翻译是一个消耗能量的过程，必须在细胞和生物体水平上进行微调，以匹配资源的可用性。雷帕霉素激酶（TOR）的靶标是响应环境线索的大范围生物过程的关键调节剂。在本研究中，我们研究了TOR失活对拟南芥不同水平核糖体蛋白表达和调控的影响，即从转录组学到磷酸化蛋白质组学。TOR失活导致编码塑性核糖体蛋白的核编码mrna的转录和翻译协同下调，这可以解释TOR沉默植物的褪绿表型。我们在这组基因的5 ‘非翻译区（UTRs）中发现了一个与5 ’末端寡聚嘧啶基序相关的保守序列，已知该序列在其他真核生物中具有TOR激酶的翻译调节作用。此外，TOR失活后核糖体部分的磷酸化蛋白质组学分析显示，40S核糖体蛋白S6 (RPS6) c端区域的保守Ser240残基磷酸化水平较低。使用特异性识别RPS6中磷酸化的Ser240的抗体，Western blot分析证实了这些结果。最后，利用该抗体跟踪植物中TOR的活性。因此，我们的研究结果揭示了TOR激酶对植物核糖体基因和蛋白质的多级调控。
Protein translation is an energy consuming process that has to be fine-tuned at both the cell and organism levels to match the availability of resources. The target of rapamycin kinase (TOR) is a key regulator of a large range of biological processes in response to environmental cues. In this study, we have investigated the effects of TOR inactivation on the expression and regulation of Arabidopsis ribosomal proteins at different levels of analysis, namely from transcriptomic to phosphoproteomic. TOR inactivation resulted in a coordinated down-regulation of the transcription and translation of nuclear-encoded mRNAs coding for plastidic ribosomal proteins, which could explain the chlorotic phenotype of the TOR silenced plants. We have identified in the 5′ untranslated regions (UTRs) of this set of genes a conserved sequence related to the 5′ terminal oligopyrimidine motif, which is known to confer translational regulation by the TOR kinase in other eukaryotes. Furthermore, the phosphoproteomic analysis of the ribosomal fraction following TOR inactivation revealed a lower phosphorylation of the conserved Ser240 residue in the C-terminal region of the 40S ribosomal protein S6 (RPS6). These results were confirmed by Western blot analysis using an antibody that specifically recognizes phosphorylated Ser240 in RPS6. Finally, this antibody was used to follow TOR activity in plants. Our results thus uncover a multi-level regulation of plant ribosomal genes and proteins by the TOR kinase.
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影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
5.8
作者：
Craig, R;Beavis, RC
通讯作者：
Beavis, RC
影响因子：
64.8
作者：
Hsieh, Andrew C.;Liu, Yi;Edlind, Merritt P.;Ingolia, Nicholas T.;Janes, Matthew R.;Sher, Annie;Shi, Evan Y.;Stumpf, Craig R.;Christensen, Carly;Bonham, Michael J.;Wang, Shunyou;Ren, Pingda;Martin, Michael;Jessen, Katti;Feldman, Morris E.;Weissman, Jonathan S.;Shokat, Kevan M.;Rommel, Christian;Ruggero, Davide
通讯作者：
Ruggero, Davide
DOI：
10.1126/science.1199498
发表时间：
2011-06-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hsu PP;Kang SA;Rameseder J;Zhang Y;Ottina KA;Lim D;Peterson TR;Choi Y;Gray NS;Yaffe MB;Marto JA;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
3.4
作者：
Allet, N;Barrillat, N;Zwahlen, C
通讯作者：
Zwahlen, C