Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens.
Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens.
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DOI：
10.1111/j.1365-2559.2010.03503.x
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发表时间：
2010-03
期刊：
影响因子：
6.4
通讯作者：
Mollerup J
中科院分区：
文献类型：
--
作者：
García-Caballero T;Grabau D;Green AR;Gregory J;Schad A;Kohlwes E;Ellis IO;Watts S;Mollerup J
García-Caballo T，Grabau D，Green A R，Gregory J，Schad A，Kohlwes E，Ellis IO，Watts S和Mollerup J(2010)组织病理学用双色显色原位杂交检测原发性乳腺癌HER2扩增可与荧光原位杂交相媲美：欧洲一项涉及168例标本的多中心研究荧光原位杂交(FISH)可用于揭示与正确的癌症诊断和正确的治疗方案有关的几个基因组失衡。然而，FISH除了需要荧光显微镜方面的专业知识外，还需要昂贵和先进的荧光显微镜。为了确定一种新发展的双色显色原位杂交方法是否适合于FISH，我们用双色显色原位杂交和FISH分析了168例乳腺癌标本中人表皮生长因子受体2基因(HER2)的扩增水平，并对结果进行了比较。我们发现FISH确定的HER2状态与双色CISH的HER2状态100%一致。此外，我们观察到，与FISH方案相比，在双色CISH中用于评分幻灯片的时间显著减少了28%。HER2蛋白状态与双色CISH或FISH的符合率相同，总体符合率为96.8%。HER2/着丝粒17基因比率与FISH的相关性达到极显著水平，总相关系数(ρ)为0.96.我们得出结论，在分析原发性乳腺癌的HER2状态时，双色CISH和明亮视野显微镜是FISH的极佳替代品。
García-Caballero T, Grabau D, Green A R, Gregory J, Schad A, Kohlwes E, Ellis I O, Watts S & Mollerup J (2010) Histopathology56, 472–480 Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a suitable alternative to FISH, we analysed the human epidermal growth factor receptor 2 gene (HER2) amplification level of 168 breast cancer specimens using dual-colour CISH and FISH and compared the results. We found 100% agreement between HER2 status determined by FISH and dual-colour CISH. Furthermore, we observed that the time used to score slides was significantly reduced by 28% in dual-colour CISH compared with the FISH protocol. Concordance between HER2 protein status and dual-colour CISH or FISH was equally good with an overall agreement of 96.8%. Correlation between the HER2/centromere 17 gene ratios obtained with dual-colour CISH and FISH was highly significant with an overall correlation coefficient (ρ) of 0.96. We conclude that dual-colour CISH and bright field microscopy are excellent alternatives to FISH when analysing the HER2 status of primary breast cancer.
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