Oligomer Formation Propensities of Dimeric Bundle Peptides Correlate with Cell Penetration Abilities.
Oligomer Formation Propensities of Dimeric Bundle Peptides Correlate with Cell Penetration Abilities.
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DOI：
10.1021/acscentsci.8b00262
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发表时间：
2018-07-25
影响因子：
18.2
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Hyun S;Lee Y;Jin SM;Cho J;Park J;Hyeon C;Kim KS;Lee Y;Yu J
合成了由两个二硫键连接的两亲性二聚肽LK-3和相关异构体束，并研究了它们的细胞穿透能力。使用尺寸排阻色谱和动态光散射进行的测量表明，LK-3 及其异构体形成细胞穿透低聚物。对各种类型的肽异构体进行的计算阐明了二聚体的两亲性特征与细胞渗透能力之间的强相关性。结果表明，LK-3 和相关束二聚体的两亲性决定了它们的寡聚倾向，而寡聚倾向又决定了它们的细胞穿透能力。本研究中的观察结果提供了有关细胞摄取 LK-3 机制的详细信息，并提出了细胞穿透两亲肽纳米颗粒形成早期阶段的合理见解。 LK-3是一种两亲性二聚肽，具有良好的细胞穿透能力。计算表明，LK-3 的两亲性决定了其寡聚化和细胞穿透能力。
LK-3, an amphipathic dimeric peptide linked by two disulfide bonds, and related isomeric bundles were synthesized, and their cell penetrating abilities were investigated. The measurements using size exclusion chromatography and dynamic light scattering show that LK-3 and its isomers form cell penetrating oligomers. Calculations, performed for various types of peptide isomers, elucidate a strong correlation between the amphipathic character of dimers and cell penetration ability. The results suggest that the amphipathicities of LK-3 and related bundle dimers are responsible for their oligomerization propensities which in turn determine their cell penetrating abilities. The observations made in this study provide detailed information about the mechanism of cell uptake of LK-3 and suggest a plausible insight of the early stage of nanoparticle formation of the cell penetrating amphipathic peptides. LK-3, an amphipathic dimeric peptide, shows good cell penetrating ability. Calculations show that the amphipathicity of LK-3 is responsible for its oligomerization and the cell penetrating abilities.
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影响因子：
13.3
作者：
dos Santos, Tiago;Varela, Juan;Dawson, Kenneth A.
通讯作者：
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
38.3
作者：
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通讯作者：
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