Optical mapping of optogenetically shaped cardiac action potentials.
Optical mapping of optogenetically shaped cardiac action potentials.
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DOI：
10.1038/srep06125
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发表时间：
2014-08-19
影响因子：
4.6
通讯作者：
Yue DT
中科院分区：
文献类型：
--
作者：
Park SA;Lee SR;Tung L;Yue DT
Light-mediated silencing and stimulation of cardiac excitability, an important complement to electrical stimulation, promises important discoveries and therapies. To date, cardiac optogenetics has been studied with patch-clamp, multielectrode arrays, video microscopy, and an all-optical system measuring calcium transients. The future lies in achieving simultaneous optical acquisition of excitability signals and optogenetic control, both with high spatio-temporal resolution. Here, we make progress by combining optical mapping of action potentials with concurrent activation of channelrhodopsin-2 (ChR2) or halorhodopsin (eNpHR3.0), via an all-optical system applied to monolayers of neonatal rat ventricular myocytes (NRVM). Additionally, we explore the capability of ChR2 and eNpHR3.0 to shape action-potential waveforms, potentially aiding the study of short/long QT syndromes that result from abnormal changes in action potential duration (APD). These results show the promise of an all-optical system to acquire action potentials with precise temporal optogenetics control, achieving a long-sought flexibility beyond the means of conventional electrical stimulation.
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影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
16.2
作者：
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Pieribone VA
DOI：
10.1161/circep.111.964247
发表时间：
2011-10
期刊：
Circulation. Arrhythmia and electrophysiology
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通讯作者：
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影响因子：
48
作者：
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通讯作者：
Sasse, Philipp