Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data
Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data
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大黄鱼TLR5和TLR13基因的分子特征及进化分析
DOI：
10.1155/2020/4895037
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发表时间：
2020-12-09
影响因子：
2.9
通讯作者：
Ogura A
中科院分区：
文献类型：
--
作者：
Jiang L;Pei L;Wang P;Liu L;Li G;Liu B;Lǚ Z;Hiromasa T;Pan H;Ogura A
TLRs （toll样受体）在宿主防御病原体中是必不可少的。TLR5有两种类型，即膜形式的TLR5 （TLR5M）和可溶性形式的TLR5 (TLR5S)，它们在鞭毛蛋白反应中起着至关重要的作用。TLR13是一种定位于核内体的TLR，可识别内部微生物（包括病毒、细菌和真菌）释放的核酸。本文对大黄鱼TLR5 （TLR5S）和TLR13的全长编码序列（CDS）、蛋白结构、免疫应答和亚细胞定位进行了研究。这些tlr与其他鱼类tlr具有较高的序列同源性，同时也具有各自的特征；qtPCR检测结果发现，这三个基因在所有检测组织中均组成性表达：TLR5M在脾脏和肝脏中高表达；TLR13在肾、肝、脾高表达。在肝、脾和肾的副溶血性弧菌刺激后，tlr上调。免疫荧光染色显示TLR5M定位于细胞质，而TLR5S和TLR13定位于核内体。进化分析表明，TLR13与TLR11、19、20、21、22聚在一起，而TLR5与TLR3聚在一起；这些结果表明，TLR在硬骨鱼抵御细菌感染中起着至关重要的作用，并进一步加深了我们对TLR在硬骨鱼先天免疫中的作用的认识。
TLRs (Toll-like receptors) are essential in host defense against pathogens. There are two types of TLR5, namely, membrane form of TLR5 (TLR5M) and soluble form of TLR5 (TLR5S), both of which perform a crucial role in flagellin response. TLR13 is a TLR that localizes to endosomes and recognizes nucleic acids released by internal microorganisms, including viruses, bacteria, and fungi. Here, the full-length coding sequence (CDS), protein structure, and immune response and subcellular localization of TLR5 (TLR5S) and TLR13 were characterized in large yellow croaker (Larimichthys crocea). These TLRs share high sequence homology with other ichthyic TLRs, while also having their own characters; qtPCR was determined and the results found that the three genes were constitutively expressed in all examined tissues: TLR5M was highly expressed in the spleen and liver; TLR13 expression was high in the kidney, liver, and spleen. And TLRs were upregulated following stimulation with Vibrio parahaemolyticus in the liver, spleen, and kidney. Immunofluorescence staining revealed that TLR5M were localized in the cytoplasm, while TLR5S and TLR13 were in the endosome. The evolutionary analysis has shown that TLR13 was clustered with TLR11, 19, 20, 21, and 22, while TLR5 and TLR3 were classified into a group; these results suggest that TLRs are vital in the defense of L. crocea against bacterial infection and further increase our understanding of TLR function in innate immunity in teleosts.
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影响因子：
4.7
作者：
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