Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice.
Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice.
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人参皂苷 Rb1 治疗可减轻小鼠肠缺血再灌注损伤后的肺部炎症细胞因子释放和组织损伤
DOI：
10.1155/2015/843721
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发表时间：
2015
影响因子：
--
通讯作者：
Xia ZY
中科院分区：
文献类型：
--
作者：
Jiang Y;Zhou Z;Meng QT;Sun Q;Su W;Lei S;Xia Z;Xia ZY
目标。肠缺血再灌注(II/R)损伤在远隔器官功能障碍中起关键作用，如肺损伤，该损伤与核因子红系2相关因子2(Nrf2)/血红素氧合酶1(HO-1)信号通路有关。本研究探讨人参皂苷Rb1是否通过Nrf2/HO-1途径减轻缺血/再灌注所致肺损伤。方法：研究方法。雄性C57BL/6J小鼠结扎肠系膜上动脉45 ，再灌注2小时，造成I/R损伤。再灌流前给予人参皂苷Rb1，同时给予或不给予全反式维甲酸(全反式维甲酸，Nrf2/ARE信号通路的抑制剂)。肺组织中丙二醛、白介素6、肿瘤坏死因子α水平升高，超氧化物歧化酶、白介素10水平降低。人参皂苷Rb1可减轻肺组织损伤，降低肺组织肿瘤坏死因子-α和丙二醛水平，降低肺湿重/干重比。有趣的是，人参皂苷Rb1可促进II/R所致肺组织中Nrf2和HO-1表达的增加。这些变化均可被ATRA抑制或阻止。结论。人参皂苷Rb1可通过激活Nrf2/HO-1途径减轻缺血/再灌注肺损伤。
Objective. Intestinal ischemia reperfusion (II/R) injury plays a critical role in remote organ dysfunction, such as lung injury, which is associated with nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. In the present study, we tested whether ginsenoside Rb1 attenuated II/R induced lung injury by Nrf2/HO-1 pathway. Methods. II/R injury was induced in male C57BL/6J mice by 45 min of superior mesenteric artery (SMA) occlusion followed by 2 hours of reperfusion. Ginsenoside Rb1 was administrated prior to reperfusion with or without ATRA (all-transretinoic acid, the inhibitor of Nrf2/ARE signaling pathway) administration before II/R. Results. II/R induced lung histological injury, which is accompanied with increased levels of malondialdehyde (MDA), interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) α but decreased levels of superoxide dismutase (SOD) and IL-10 in the lung tissues. Ginsenoside Rb1 reduced lung histological injury and the levels of TNF-α and MDA, as well as wet/dry weight ratio. Interestingly, the increased Nrf2 and HO-1 expression induced by II/R in the lung tissues was promoted by ginsenoside Rb1 treatment. All these changes could be inhibited or prevented by ATRA. Conclusion. Ginsenoside Rb1 is capable of ameliorating II/R induced lung injuries by activating Nrf2/HO-1 pathway.
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影响因子：
20.1
作者：
PEARCE, ML;YAMASHITA, J;BEAZELL, J
通讯作者：
BEAZELL, J
影响因子：
5.8
作者：
Chia AJ;Goldring CE;Kitteringham NR;Wong SQ;Morgan P;Park BK
通讯作者：
Park BK
影响因子：
24
作者：
Pachori, AS;Melo, LG;Dzau, VJ
通讯作者：
Dzau, VJ
影响因子：
4.3
作者：
Smolinski, AT;Pestka, JJ
通讯作者：
Pestka, JJ
影响因子：
2.2
作者：
Liu, Ke-Xuan;Li, Yun-Sheng;Li, Yi
通讯作者：
Li, Yi