Scalable and DiI-compatible optical clearance of the mammalian brain.
Scalable and DiI-compatible optical clearance of the mammalian brain.
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哺乳动物大脑的可扩展且兼容 Dil 的光学间隙
DOI：
10.3389/fnana.2015.00019
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发表时间：
2015
影响因子：
2.9
通讯作者：
Jiang T
中科院分区：
文献类型：
--
作者：
Hou B;Zhang D;Zhao S;Wei M;Yang Z;Wang S;Wang J;Zhang X;Liu B;Fan L;Li Y;Qiu Z;Zhang C;Jiang T
有效的光学清晰度是全脑成像的基础。特别是，对于亲脂性示踪剂标记的神经束的成像，需要清除大脑而不破坏膜。依靠果糖溶液的上升梯度，SeeDB可以实现小鼠大脑的足够透明度，同时确保质膜保持完整。然而，由于饱和果糖溶液的极高粘度，将这种方法推广到更大的哺乳动物大脑是具有挑战性的。在这里，我们报告了一种SeeDB衍生的光学透明方法，称为水果，它利用果糖和尿素的鸡尾酒。正如在成年小鼠大脑中证明的那样，这两种高度水溶性的清除剂的组合在清除方面发挥了协同作用。更重要的是，最终的水果溶液具有低粘度，从而通过动脉灌流产生整个成年兔脑的透明度，这是饱和果糖溶液无法实现的。除了与增强型黄色荧光蛋白良好的兼容性外，该鸡尾酒还保留了亲脂性示踪剂DII的荧光。这项工作提供了一种体积无关的光学清除方法，该方法保留了SeeDB的优点，特别是与亲脂性示踪剂的兼容性。
Efficient optical clearance is fundamental for whole brain imaging. In particular, clearance of the brain without membrane damage is required for the imaging of lipophilic tracer-labeled neural tracts. Relying on an ascending gradient of fructose solutions, SeeDB can achieve sufficient transparency of the mouse brain while ensuring that the plasma membrane remains intact. However, it is challenging to extend this method to larger mammalian brains due to the extremely high viscosity of the saturated fructose solution. Here we report a SeeDB-derived optical clearing method, termed FRUIT, which utilizes a cocktail of fructose and urea. As demonstrated in the adult mouse brain, combination of these two highly water-soluble clearing agents exerts a synergistic effect on clearance. More importantly, the final FRUIT solution has low viscosity so as to produce transparency of the whole adult rabbit brain via arterial perfusion, which is impossible to achieve with a saturated fructose solution. In addition to good compatibility with enhanced yellow fluorescent protein, the cocktail also preserves the fluorescence of the lipophilic tracer DiI. This work provides a volume-independent optical clearing method which retains the advantages of SeeDB, particularly compatibility with lipophilic tracers.
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影响因子：
1.9
作者：
HALE, GM;QUERRY, MR
通讯作者：
QUERRY, MR
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
14.8
作者：
通讯作者：
--
影响因子：
64.8
作者：
通讯作者：
--
微光学断层扫描获得小鼠大脑的高分辨率图谱
影响因子：
56.9
作者：
Li, Anan;Gong, Hui;Luo, Qingming
通讯作者：
Luo, Qingming