Insights into environmental controls on microbial communities in a continental serpentinite aquifer using a microcosm-based approach.
Insights into environmental controls on microbial communities in a continental serpentinite aquifer using a microcosm-based approach.
复制标题
DOI：
10.3389/fmicb.2014.00604
复制
发表时间：
2014
影响因子：
5.2
通讯作者：
Schrenk MO
中科院分区：
文献类型：
--
作者：
Crespo-Medina M;Twing KI;Kubo MD;Hoehler TM;Cardace D;McCollom T;Schrenk MO
与蛇纹岩作用相关的地球化学反应改变了循环流体中溶解有机化合物的组成，并可能释放出地幔源碳和还原力，以支持地下微生物群落。先前的研究已经确定了来自伯克霍尔德目的Betaproteobacteria和来自梭状芽胞杆菌目的细菌是蛇纹岩宿主微生物组的关键组成部分，但是对它们的代谢能力或生长特性的了解有限。为了更好地描述微生物群落、它们的代谢和限制它们活动的因素，在2013年3月和8月的考察期间，我们设计了微观世界实验，并从加利福尼亚北部海岸范围蛇绿岩微生物观测站（CROMO）的几个监测井中收集了液体。在氢气气氛和各种碳源（二氧化碳、甲烷、醋酸酯和甲酸酯）中开始培养，有或没有添加营养物质和电子受体。通过直接显微镜计数监测生长；在3个月孵育结束时评估DNA产量和群落组成。结果表明，在大多数情况下，乙酸盐和甲烷的添加有利于细菌的生长，而营养物质和电子受体的添加对微生物的生长没有显著影响，表明没有营养或氧化剂限制。然而，硫的添加导致了不同的群落组成。孵育结束时的优势生物与Dethiobacter sp.和Comamonadaceae家族密切相关，这在非培养基因测序调查中也很突出。这些实验提供了对蛇纹岩地下环境的生物地球化学动力学的初步见解之一，并将促进在蛇纹岩生态系统中追踪微生物活动的实验。
Geochemical reactions associated with serpentinization alter the composition of dissolved organic compounds in circulating fluids and potentially liberate mantle-derived carbon and reducing power to support subsurface microbial communities. Previous studies have identified Betaproteobacteria from the order Burkholderiales and bacteria from the order Clostridiales as key components of the serpentinite–hosted microbiome, however there is limited knowledge of their metabolic capabilities or growth characteristics. In an effort to better characterize microbial communities, their metabolism, and factors limiting their activities, microcosm experiments were designed with fluids collected from several monitoring wells at the Coast Range Ophiolite Microbial Observatory (CROMO) in northern California during expeditions in March and August 2013. The incubations were initiated with a hydrogen atmosphere and a variety of carbon sources (carbon dioxide, methane, acetate, and formate), with and without the addition of nutrients and electron acceptors. Growth was monitored by direct microscopic counts; DNA yield and community composition was assessed at the end of the 3 month incubation. For the most part, results indicate that bacterial growth was favored by the addition of acetate and methane, and that the addition of nutrients and electron acceptors had no significant effect on microbial growth, suggesting no nutrient- or oxidant-limitation. However, the addition of sulfur amendments led to different community compositions. The dominant organisms at the end of the incubations were closely related to Dethiobacter sp. and to the family Comamonadaceae, which are also prominent in culture-independent gene sequencing surveys. These experiments provide one of first insights into the biogeochemical dynamics of the serpentinite subsurface environment and will facilitate experiments to trace microbial activities in serpentinizing ecosystems.
登录
查看更多内容
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.4
作者：
Brazelton, William J.;Morrill, Penny L.;Schrenk, Matthew O.
通讯作者：
Schrenk, Matthew O.
影响因子：
4.4
作者：
Huber, JA;Butterfield, DA;Baross, JA
通讯作者：
Baross, JA
影响因子：
4.4
作者：
Schrenk, MO;Kelley, DS;Baross, JA
通讯作者：
Baross, JA
影响因子：
3.9
作者：
ABRAJANO, TA;STURCHIO, NC;STEVENS, CM
通讯作者：
STEVENS, CM