Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics.
Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics.
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DOI：
10.1038/srep38915
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发表时间：
2016-12-12
影响因子：
4.6
通讯作者：
Setubal JC
中科院分区：
文献类型：
--
作者：
Antunes LP;Martins LF;Pereira RV;Thomas AM;Barbosa D;Lemos LN;Silva GM;Moura LM;Epamino GW;Digiampietri LA;Lombardi KC;Ramos PL;Quaggio RB;de Oliveira JC;Pascon RC;Cruz JB;da Silva AM;Setubal JC
堆肥是一种有前途的新生物和热稳定酶的来源，可能有助于环境管理和工业过程。在这里，我们提出的结果宏基因组和metatranscriptomic为基础的分析，在圣保罗动物园的大型堆肥操作。这种堆肥表现出持续的嗜热特征（50 °C至75 °C），这似乎排除了真菌活性。我们研究的主要新奇在于将时间序列采样与鸟枪DNA、16 S rRNA基因扩增和元转录组高通量测序相结合，从而能够对该生态系统中的微生物群落结构、动态和功能进行前所未有的详细观察。时间序列数据表明，翻堆过程对堆肥微生物群有很大的影响，在一定程度上恢复了过程开始时的种群分布;木质纤维素生物质解构是协同和顺序发生的，半纤维素优先降解纤维素和木质素。此外，我们的测序数据允许之前在生物质降解环境中发现的五种细菌物种和一种新的生物降解细菌物种（可能是芽孢杆菌目中的一个新属）的近完整的基因组重建。所提供的数据和分析是嗜热堆肥微生物学的额外调查的丰富来源。
Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomic- and metatranscriptomic-based analyses of a large composting operation in the São Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 °C to 75 °C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process; and that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.2
作者：
Dees, PM;Ghiorse, WC
通讯作者：
Ghiorse, WC
影响因子：
7.8
作者：
Cragg SM;Beckham GT;Bruce NC;Bugg TD;Distel DL;Dupree P;Etxabe AG;Goodell BS;Jellison J;McGeehan JE;McQueen-Mason SJ;Schnorr K;Walton PH;Watts JE;Zimmer M
通讯作者：
Zimmer M
影响因子：
4.2
作者：
Chandna P;Nain L;Singh S;Kuhad RC
通讯作者：
Kuhad RC
影响因子：
6
作者：
DROFFNER, ML;BRINTON, WF;EVANS, E
通讯作者：
EVANS, E