Assessment of bacterial diversity during composting of agricultural byproducts.
Assessment of bacterial diversity during composting of agricultural byproducts.
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DOI：
10.1186/1471-2180-13-99
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发表时间：
2013-05-07
期刊：
影响因子：
4.2
通讯作者：
Kuhad RC
中科院分区：
文献类型：
--
作者：
Chandna P;Nain L;Singh S;Kuhad RC
堆肥是可生物降解物质的微生物分解过程，受物理化学、生理和微生物等多种因素的制约。微生物群落(细菌、放线菌和真菌)在堆肥过程中的重要性是公认的。然而，堆肥过程中的微生物多样性可能会因堆肥材料和营养补充剂的不同而不同。因此，有必要研究不同的农副产品如麦皮、米皮、稻壳以及剪草和膨胀剂在堆肥过程中的微生物多样性。在此，我们试图评估农业副产品堆肥过程中可培养细菌的多样性。在此过程中，通过分离最突出的细菌来评估可培养细菌的多样性。细菌数量在中温阶段达到最大，在高温阶段有所下降，在冷却和成熟阶段进一步下降。堆肥细菌数量在105~109cfu g-1之间。对优势菌进行生化鉴定和16S rRNA基因测序。分离的革兰氏阳性菌和革兰氏阴性菌分别属于伯克霍尔德菌、肠杆菌、放线杆菌和芽孢杆菌，其中包括葡萄球菌属、沙雷菌属、克雷伯氏菌属、肠杆菌属、土杆菌属、柯氏杆菌属、微杆菌属、嗜酸杆菌属和单胞菌属。从农副产品制成的堆肥中还首次鉴定出Kocuria属、微杆菌属、Acidovorax属、Comamonas属和一些芽孢杆菌新种。在堆肥过程中使用适当的氮肥改良剂和膨胀剂，堆肥质量较好。这种基于培养的策略使我们能够首次从农副产品堆肥中分离到一些新的细菌菌株，如Kocuria，Micro杆菌，Acidovorax和Comamonas。这些细菌可以作为潜在的堆肥接种剂，加速堆肥进程。
Composting is microbial decomposition of biodegradable materials and it is governed by physicochemical, physiological and microbiological factors. The importance of microbial communities (bacteria, actinomycetes and fungi) during composting is well established. However, the microbial diversity during composting may vary with the variety of composting materials and nutrient supplements. Therefore, it is necessary to study the diversity of microorganisms during composting of different agricultural byproducts like wheat bran, rice bran, rice husk, along with grass clippings and bulking agents. Here it has been attempted to assess the diversity of culturable bacteria during composting of agricultural byproducts. The culturable bacterial diversity was assessed during the process by isolating the most prominent bacteria. Bacterial population was found to be maximum during the mesophilic phase, but decreased during the thermophilic phase and declined further in the cooling and maturation phase of composting. The bacterial population ranged from 105 to 109 cfu g-1 compost. The predominant bacteria were characterized biochemically, followed by 16S rRNA gene sequencing. The isolated strains, both Gram-positive and Gram-negative groups belonged to the order Burkholderiales, Enterobacteriales, Actinobacteriales and Bacillales, which includes genera e.g. Staphylococcus, Serratia, Klebsiella, Enterobacter, Terribacillus, Lysinibacillus Kocuria, Microbacterium, Acidovorax and Comamonas. Genera like Kocuria, Microbacterium, Acidovorax, Comamonas and some new species of Bacillus were also identified for the first time from the compost made from agricultural byproducts. The use of appropriate nitrogen amendments and bulking agents in composting resulted in good quality compost. The culture based strategy enabled us to isolate some novel bacterial isolates like Kocuria, Microbacterium, Acidovorax and Comamonas first time from agro-byproducts compost. These bacteria can be used as potential compost inoculants for accelerating composting process.
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影响因子：
4.2
作者：
Dees, PM;Ghiorse, WC
通讯作者：
Ghiorse, WC
DOI：
10.1099/ijs.0.012369-0
发表时间：
2010-07-01
影响因子：
2.8
作者：
Denizci, Aziz Akin;Kazan, Dilek;Erarslan, Altan
通讯作者：
Erarslan, Altan
影响因子：
3.5
作者：
Bandounas L;Wierckx NJ;de Winde JH;Ruijssenaars HJ
通讯作者：
Ruijssenaars HJ
影响因子：
5
作者：
Fracchia, Letizia;Dohrmann, Anja B.;Tebbe, Christoph C.
通讯作者：
Tebbe, Christoph C.
DOI：
10.1073/pnas.89.12.5685
发表时间：
1992-06-15
影响因子：
11.1
作者：
DELONG, EF
通讯作者：
DELONG, EF