Attenuation of myogenic orofacial nociception and mechanical hypersensitivity by viral mediated enkephalin overproduction in male and female rats.
Attenuation of myogenic orofacial nociception and mechanical hypersensitivity by viral mediated enkephalin overproduction in male and female rats.
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DOI：
10.1186/s12883-015-0285-5
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发表时间：
2015-03-15
期刊：
影响因子：
2.6
通讯作者：
Bellinger LL
中科院分区：
文献类型：
--
作者：
Kramer PR;Umorin M;Bellinger LL
临床研究已经测试了一种工程疱疹病毒的使用来治疗疼痛。我们假设皮下注射表达脑啡肽的工程疱疹病毒将通过中枢机制减轻雄性和雌性大鼠的口面部伤害性和超敏反应。在咬肌腱结扎前72小时，雌雄大鼠口周皮下注射疱疹病毒，对照组不感染病毒或不结扎。测定脑啡肽表达，用von Frey细丝试验和进食时间分别测定机械超敏反应和伤害性反应。给注射脑啡肽表达病毒的大鼠注射纳洛酮或甲碘化纳洛酮，以检测脑啡肽是外周作用还是中枢作用。结扎显著延长了进食时间，降低了对von Frey细丝的反应阈值18天。脑啡肽转基因感染至少在11天内显着降低了这种反应，但仅在雄性大鼠中。注射病毒后，脑啡肽在支配口区、三叉神经节和三叉神经尾侧核的精神神经中的表达显著增加，而在咬肌神经中的表达没有明显增加。纳洛酮而不是甲碘化纳洛酮逆转了脑啡肽表达病毒的反应。这些数据表明，在使用这种病毒时应该考虑性别，通过脑啡肽转基因病毒转导精神神经可以通过中枢机制减少伤害性和超敏反应。本文的在线版本(doi：10.1186/s12883-0150285-5)包含补充材料，授权用户可以使用。
Clinical studies have tested the use of an engineered herpes virus to treat pain. We hypothesized that subcutaneous injections of an engineered herpes virus that expresses enkephalin would attenuate orofacial nociception and hypersensitivity in male and female rats by a central mechanism. Herpes virus was injected subcutaneously around the mouth of male and female rats seventy-two hours before ligatures were placed on the masseter tendon, control treatment groups received either no virus or no ligature. Enkephalin expression was measured and von Frey filament testing and meal duration were utilized to measure mechanical hypersensitivity and the nociceptive response, respectively. Naloxone or naloxone methiodide was administered to rats injected with the enkephalin expressing virus to test if enkephalin was acting peripherally or centrally. Ligature significantly lengthened meal duration and reduced the threshold to von Frey filaments for 18 days. Infection with the enkephalin transgene significantly decreased this response for at least 11 days but only in male rats. Virus injection significantly increased expression of enkephalin in the mental nerve that innervates the mouth region, the trigeminal ganglia and the trigeminal nucleus caudalis but no increase was observed in the masseter nerve after virus injection. Naloxone but not naloxone methiodide reversed the response to the enkephaline expressing virus. The data suggests that sex should be a considered when using this virus and that viral transfection of the mental nerve with an enkephalin transgene can reduce nociception and hypersensitivity through a central mechanism. The online version of this article (doi:10.1186/s12883-015-0285-5) contains supplementary material, which is available to authorized users.
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影响因子：
3.4
作者：
Amandusson, A;Hermanson, O;Blomqvist, A
通讯作者：
Blomqvist, A
影响因子：
2.7
作者：
Bernal, Scott A.;Morgan, Michael M.;Craft, Rebecca M.
通讯作者：
Craft, Rebecca M.
影响因子：
5.3
作者：
Claiborne, Jomo;Nag, Subodh;Mokha, Sukhbir S.
通讯作者：
Mokha, Sukhbir S.
影响因子：
2.9
作者：
Bellinger, Larry L.;Spears, Robert;Kramer, Phillip R.
通讯作者：
Kramer, Phillip R.
DOI：
10.1016/j.ejpain.2011.03.007
发表时间：
2011-10
期刊：
European journal of pain (London, England)
影响因子：
--
作者：
Chattopadhyay M;Mata M;Fink DJ
通讯作者：
Fink DJ