The bcp gene in the bcp-recA-vimA-vimE-vimF operon is important in oxidative stress resistance in Porphyromonas gingivalis W83.
The bcp gene in the bcp-recA-vimA-vimE-vimF operon is important in oxidative stress resistance in Porphyromonas gingivalis W83.
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DOI：
10.1111/j.2041-1014.2010.00596.x
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发表时间：
2011-03
影响因子：
3.7
通讯作者：
Fletcher HM
中科院分区：
文献类型：
--
作者：
Johnson NA;McKenzie RM;Fletcher HM
牙龈卟啉单胞菌克服牙周袋炎症环境中氧化应激的能力对其生存至关重要。我们以前已经证明，recA基因座，携带细菌铁蛋白共迁移蛋白（bcp）基因，并具有独特的遗传结构，在牙龈卟啉单胞菌的毒力调节和抗氧化应激中发挥作用。为了进一步表征bcp基因，其被证实是bcp-recA-vimA-vimE-vimF操纵子的一部分，我们通过等位基因交换创建了牙龈卟啉单胞菌bcp缺陷型等基因突变体（FLL 302）。与野生型相比，FLL 302具有相似的生长速率、黑色色素沉着、β-溶血和紫外线敏感性。虽然牙龈菌蛋白酶活性的分布没有变化，但与野生型相比，突变株中Arg-X和Lys-X的活性均降低了30%。当暴露于0.25 mM过氧化氢时，牙龈卟啉单胞菌FLL 302比野生型更敏感。此外，克隆的牙龈卟啉单胞菌bcp基因增加了bcp缺陷型大肠杆菌突变体对0.25 mM过氧化氢的抗性。与野生型相比，突变体的耐氧性也有所降低。牙龈卟啉单胞菌FLL 302和野生型菌株在小鼠毒力模型中具有相似的毒力特征。这些观察结果表明，bcp基因可能在抗氧化应激中发挥作用，但在牙龈卟啉单胞菌的致病潜力中具有降低的功能意义。
The ability of Porphyromonas gingivalis to overcome oxidative stress in the inflammatory environment of the periodontal pocket is critical for its survival. We have previously demonstrated that the recA locus, which carries the bacterioferritin co-migratory protein (bcp) gene and has a unique genetic architecture, plays a role in virulence regulation and oxidative stress resistance in P. gingivalis. To further characterize the bcp gene, which was confirmed to be part of the bcp-recA-vimA-vimE-vimF operon, we created a P. gingivalis bcp-defective isogenic mutant (FLL302) by allelic exchange. Compared with the wild-type, FLL302 had a similar growth rate, black pigmentation, β-hemolysis and UV sensitivity. Although there was no change in the distribution of gingipain activity, there was a 30% reduction in both Arg-X and Lys-X activities in the mutant strain compared with the wild-type. When exposed to 0.25 mM hydrogen peroxide, P. gingivalis FLL302 was more sensitive than the wild-type. In addition, the cloned P. gingivalis bcp gene increased resistance to 0.25 mM hydrogen peroxide in a bcp-defective Escherichia coli mutant. The mutant also demonstrated decreased aerotolerance when compared with the wild-type. Porphyromonas gingivalis FLL302 and the wild-type strain had similar virulence profiles in a mouse model of virulence. These observations suggest that the bcp gene may play a role in oxidative stress resistance but has a decreased functional significance in the pathogenic potential of P. gingivalis.
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影响因子：
3.1
作者：
Abaibou, H;Chen, Z;Fletcher, HM
通讯作者：
Fletcher, HM
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
7.6
作者：
AMANO, A;TAMAGAWA, H;TSUNEMITSU, A
通讯作者：
TSUNEMITSU, A
影响因子：
7.6
作者：
GENCO, RJ;SLOTS, J
通讯作者：
SLOTS, J
影响因子：
3.2
作者：
Baillon, MLA;van Vliet, AHM;Penn, CW
通讯作者：
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