A prototype tobacco-associated oral squamous cell carcinoma classifier using RNA from brush cytology.
A prototype tobacco-associated oral squamous cell carcinoma classifier using RNA from brush cytology.
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DOI：
10.1111/jop.12068
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Adami GR
中科院分区：
文献类型：
--
作者：
Kolokythas A;Bosman MJ;Pytynia KB;Panda S;Sroussi HY;Dai Y;Schwartz JL;Adami GR
鳞状细胞癌（OSCC）形式的口腔癌通常在治疗复杂且可能无法治愈的晚期检测到。手术活检的需要可能导致诊断延迟，并妨碍早期发现。对手术获得的肿瘤样本的RNA的多项研究揭示了许多与这种疾病差异表达的基因。我们试图确定，如果确定的mRNA可以作为标记物的非侵入性检测系统的口腔鳞癌使用RNA刷细胞学。与对照组相比，来自已知在头颈部鳞状细胞癌手术样本中差异表达的21个基因的mRNA水平在口腔刷细胞学样本中显示为可定量的。这些mRNA在来自烟草/槟榔使用者的14个肿瘤和20个非恶性刷状细胞学样本的训练集中进行定量。另外两种mRNA的测量和使用支持向量机的分析产生了用于这些癌症的类别预测的算法。该OSCC分类器基于刷拭细胞学RNA中5种mRNA的水平，基于留一法交叉验证，最初在区分OSCC与良性口腔粘膜病变方面显示出0.93的灵敏度和0.91的特异性。当使用的测试集的19个样本，从6个口腔鳞状细胞癌和13个非恶性口腔病变，我们发现只有一个口腔鳞状细胞癌和一个良性病变的错误分类。这表明使用刷细胞学的RNA用于早期OSCC检测的前景和这种非侵入性分类器的临床应用潜力。
Oral cancer in the form of squamous cell carcinoma (OSCC) is typically detected in advanced stages when treatment is complex and may not be curative. The need for surgical biopsy may contribute to delays in diagnosis and impede early detection. Multiple studies of RNA from surgically obtained tumor samples have revealed many genes differentially expressed with this disease. We sought to determine if the identified mRNAs could be used as markers by a noninvasive detection system for OSCC using RNA from brush cytology. Levels of mRNAs from 21 genes known to be differentially expressed in head and neck squamous cell carcinoma surgical samples, compared to controls, were shown to be quantifiable in oral brush cytology samples. These mRNAs were quantified in a training set of 14 tumor and 20 nonmalignant brush cytology samples from tobacco/betel nut users. The measurement of two additional mRNAs and analysis using support vector machines produced an algorithm for class prediction of these cancers. This OSCC classifier based on the levels of 5 mRNAs in RNA from brush cytology initially showed 0.93 sensitivity and 0.91 specificity in differentiating OSCC from benign oral mucosal lesions based on leave-one-out cross-validation. When used on a test set of 19 samples from 6 OSCCs and 13 nonmalignant oral lesions we found misclassification of only one OSCC and one benign lesion. This shows the promise of using RNA from brush cytology for early OSCC detection and the potential for clinical usage of this noninvasive classifier.
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影响因子：
11.2
作者：
Spivack, SD;Hurteau, GJ;Kaminsky, LS
通讯作者：
Kaminsky, LS
影响因子：
4.8
作者：
Poate, TWJ;Buchanan, JAG;Porter, SR
通讯作者：
Porter, SR
用于早期诊断口腔鳞状细胞癌的新型多标记方法
影响因子：
--
作者：
Ries, Jutta;Mollaoglu, Nur;Nkenke, Emeka
通讯作者：
Nkenke, Emeka
影响因子：
--
作者：
Lohavanichbutr, Pawadee;Houck, John;Fan, Wenhong;Yueh, Bevan;Mendez, Eduardo;Futran, Neal;Doody, David R.;Upton, Melissa P.;Farwell, D. Gregory;Schwartz, Stephen M.;Zhao, Lue Ping;Chen, Chu
通讯作者：
Chen, Chu
影响因子：
--
作者：
Choi, Peter;Jordan, C. Diana;Chen, Chu
通讯作者：
Chen, Chu