Murine dendritic cells loaded in vitro with soluble protein prime cytotoxic T lymphocytes against tumor antigen in vivo.
Murine dendritic cells loaded in vitro with soluble protein prime cytotoxic T lymphocytes against tumor antigen in vivo.
复制标题
DOI：
10.1084/jem.183.1.317
复制
发表时间：
1996-01-01
影响因子：
15.3
通讯作者：
Colombo, MP
中科院分区：
文献类型：
--
作者：
Paglia, P;Chiodoni, C;Rodolfo, M;Colombo, MP
The priming of an immune response against a major histocompatibility complex class I-restricted antigen expressed by nonhematopoietic cells involves the transfer of that antigen to a host bone marrow-derived antigen presenting cell (APC) for presentation to CD8+ T lymphocytes. Dendritic cells (DC), as bone marrow-derived APC, are first candidates for presentation of tumor-associated antigens (TAA). The aim of this study was to see whether DC are able to prime in vivo antigen-specific cytotoxic T lymphocytes after exposure to a soluble protein antigen in vitro. Lacking a well-defined murine TAA, we took advantage of beta- galactosidase (beta-gal)-transduced tumor cell lines as a model in which beta-gal operationally functions as TAA. For in vivo priming both a DC line, transduced or not transduced with the gene coding for murine GM-CSF, and fresh bone marrow-derived DC (bm-DC), loaded in vitro with soluble beta-gal, were used. Priming with either granulocyte macrophage colony-stimulating factor-transduced DC line or fresh bm-DC but not with untransduced DC line generated CTL able to lyse beta-gal- transfected target cells. Furthermore, GM-CSF was necessary for the DC line to efficiently present soluble beta-gal as an H-2Ld-restricted peptide to a beta-gal-specific CTL clone. Data also show that a long- lasting immunity against tumor challenge can be induced using beta-gal- pulsed bm-DC as vaccine. These results indicate that effector cells can be recruited and activated in vivo by antigen-pulsed DC, providing an efficient immune reaction against tumors.
登录
查看更多内容
影响因子：
4.4
作者：
Sallusto, Federica;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
影响因子：
5.4
作者：
FLAMAND, V;SORNASSE, T;MOSER, M
通讯作者：
MOSER, M
影响因子：
3.7
作者：
MARKOWITZ, D;GOFF, S;BANK, A
通讯作者：
BANK, A
用蛋白质抗原在体外脉动的树突状细胞可以原位抗原特异性，MHC限制的T细胞。
影响因子：
15.3
作者：
Inaba, K;Metlay, J P;Crowley, M T;Steinman, R M
通讯作者：
Steinman, R M
树突状细胞直接激活CD8+细胞毒性T淋巴细胞。
影响因子：
15.3
作者：
INABA, K;YOUNG, JW;STEINMAN, RM
通讯作者：
STEINMAN, RM