Attenuation of High-Fat Diet-Induced Rat Liver Oxidative Stress and Steatosis by Combined Hydroxytyrosol- (HT-) Eicosapentaenoic Acid Supplementation Mainly Relies on HT.
Attenuation of High-Fat Diet-Induced Rat Liver Oxidative Stress and Steatosis by Combined Hydroxytyrosol- (HT-) Eicosapentaenoic Acid Supplementation Mainly Relies on HT.
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DOI：
10.1155/2018/5109503
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发表时间：
2018
影响因子：
--
通讯作者：
Videla LA
中科院分区：
文献类型：
--
作者：
Echeverría F;Valenzuela R;Bustamante A;Álvarez D;Ortiz M;Soto-Alarcon SA;Muñoz P;Corbari A;Videla LA
非酒精性脂肪性肝病（NAFLD）的药物治疗目前尚未获批。为此，本研究评估了二碳五烯酸（EPA， 50 mg/kg/天）调节肝脏脂质代谢和羟基酪醇（HT， 5 mg/kg/天）对高脂饮食（HFD， 60%脂肪，20%蛋白质和20%碳水化合物）与对照饮食（CD， 10%脂肪，20%蛋白质和70%碳水化合物）诱导的小鼠肝脏脂肪变性和氧化应激的影响。HFD诱导的肝脂肪变性(i)由EPA减少32%，氧化应激相关参数不变，Nrf2功能轻度恢复，提供抗氧化作用；（ii）由HT减少42%，同时HFD减少的谷胱甘肽状态总恢复，HFD增强的脂质过氧化和蛋白质氧化恢复42%至59%，Nrf2功能恢复，而（iii） EPA + HT联合补充可使肝脏脂肪变性减少74%。总抗氧化潜能恢复的方式与高温疗法相似。由此可见，HT + EPA联用可显著降低NAFLD的发展，其作用表现为HT与EPA作用的加和性，而HT的作用主要依赖于EPA，强化了氧化应激的影响，是肥胖肝脂肪变性的核心机制。
Pharmacological therapy for nonalcoholic fatty liver disease (NAFLD) is not approved at the present time. For this purpose, the effect of combined eicosapentaenoic acid (EPA; 50 mg/kg/day) modulating hepatic lipid metabolism and hydroxytyrosol (HT; 5 mg/kg/day) exerting antioxidant actions was evaluated on hepatic steatosis and oxidative stress induced by a high-fat diet (HFD; 60% fat, 20% protein, and 20% carbohydrates) compared to a control diet (CD; 10% fat, 20% protein, and 70% carbohydrates) in mice fed for 12 weeks. HFD-induced liver steatosis (i) was reduced by 32% by EPA, without changes in oxidative stress-related parameters and mild recovery of Nrf2 functioning affording antioxidation and (ii) was decreased by 42% by HT, concomitantly with total regain of the glutathione status diminished by HFD, 42% to 59% recovery of lipid peroxidation and protein oxidation enhanced by HFD, and regain of Nrf2 functioning, whereas (iii) combined EPA + HT supplementation elicited 74% reduction in liver steatosis, with total recovery of the antioxidant potential in a similar manner than HT. It is concluded that combined HT + EPA drastically decreases NAFLD development, an effect that shows additivity in HT and EPA effects that mainly relies on HT, strengthening the impact of oxidative stress as a central mechanism underlying liver steatosis in obesity.
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DOI：
10.2147/hmer.s125718
发表时间：
2017
期刊：
Hepatic medicine : evidence and research
影响因子：
--
作者：
Bruschi FV;Tardelli M;Claudel T;Trauner M
通讯作者：
Trauner M
影响因子：
7.3
作者：
Lau, Jennie Ka Ching;Zhang, Xiang;Yu, Jun
通讯作者：
Yu, Jun
影响因子：
25.7
作者：
Malhi, Harmeet;Kaufman, Randal J.
通讯作者：
Kaufman, Randal J.
影响因子：
5.6
作者：
Echeverría F;Ortiz M;Valenzuela R;Videla LA
通讯作者：
Videla LA
基于羟基羟基醇的药物配方对人体组成，代谢状态和基因表达的抗氧化作用：一项随机的双盲，安慰剂对照的交叉试验。
影响因子：
--
作者：
Colica C;Di Renzo L;Trombetta D;Smeriglio A;Bernardini S;Cioccoloni G;Costa de Miranda R;Gualtieri P;Sinibaldi Salimei P;De Lorenzo A
通讯作者：
De Lorenzo A