Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis.

Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis.
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srfB 的克隆和表征,srfB 是枯草芽孢杆菌中参与表面活性素产生和能力的调节基因。

DOI:
10.1128/jb.171.10.5347-5353.1989
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发表时间:
1989
影响因子:
3.2
通讯作者:
Zuber,P
Zuber,P
中科院分区:
生物学3区
文献类型:
--
作者:
Nakano,MM;Zuber,P

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Tn917插入突变srfB破坏枯草芽孢杆菌脂肽抗生素表面素的产生。根据噬菌体PBS1的转导定位,srfB位于枯草芽孢杆菌基因组的aroG和ald之间,与先前描述的表面蛋白位点sfp或srfA无关。一个srfB突变体在能力的建立上也存在缺陷。SP β噬菌体介导的互补分析表明,srfB突变体通过一个1.5千碱基对的DNA片段恢复了能力和表面素的产生。互补DNA序列显示srfB基因为comA,是一种早期能力基因,其编码的产物类似于细菌双组分调控系统的激活子类。srfB突变破坏了srfA-lacZ融合的表达,表明srfB (comA)基因产物在转录水平上积极调节表面素的产生。
A Tn917 insertion mutation srfB impairs the production of the lipopeptide antibiotic surfactin in Bacillus subtilis. srfB is located between aroG and ald in the B. subtilis genome, as determined by phage PBS1 transduction mapping, and is not linked to the previously described surfactin loci sfp or srfA. A srfB mutant was found to be also deficient in the establishment of competence. SP beta phage-mediated complementation analysis showed that both competence and surfactin production were restored in the srfB mutant by a single DNA fragment of 1.5 kilobase pairs. The sequence of the complementing DNA revealed that the srfB gene is comA, an early competence gene which codes for a product similar to that of the activator class of bacterial two-component regulatory systems. The srfB mutation impaired the expression of a srfA-lacZ fusion, suggesting that surfactin production is positively regulated at the transcriptional level by the srfB (comA) gene product.
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