Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis.
Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis.
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srfB 的克隆和表征,srfB 是枯草芽孢杆菌中参与表面活性素产生和能力的调节基因。
DOI:
10.1128/jb.171.10.5347-5353.1989
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发表时间:
1989
影响因子:
3.2
通讯作者:
Zuber,P
中科院分区:
文献类型:
--
作者:
Nakano,MM;Zuber,P
A Tn917 insertion mutation srfB impairs the production of the lipopeptide antibiotic surfactin in Bacillus subtilis. srfB is located between aroG and ald in the B. subtilis genome, as determined by phage PBS1 transduction mapping, and is not linked to the previously described surfactin loci sfp or srfA. A srfB mutant was found to be also deficient in the establishment of competence. SP beta phage-mediated complementation analysis showed that both competence and surfactin production were restored in the srfB mutant by a single DNA fragment of 1.5 kilobase pairs. The sequence of the complementing DNA revealed that the srfB gene is comA, an early competence gene which codes for a product similar to that of the activator class of bacterial two-component regulatory systems. The srfB mutation impaired the expression of a srfA-lacZ fusion, suggesting that surfactin production is positively regulated at the transcriptional level by the srfB (comA) gene product.
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DOI:
10.1042/bj2180691
发表时间:
1984
期刊:
The Biochemical journal
影响因子:
--
作者:
H. Masure;J. Head;H. M. Tice
通讯作者:
H. M. Tice
DOI:
10.1099/00221287-63-2-211
发表时间:
1970-01-01
期刊:
JOURNAL OF GENERAL MICROBIOLOGY
影响因子:
--
作者:
STAINER, DW;SCHOLTE, MJ
通讯作者:
SCHOLTE, MJ
DOI:
--
发表时间:
1980
影响因子:
11.1
作者:
J. Wolff;G. H. Cook;A. Goldhammer;S. Berkowitz
通讯作者:
S. Berkowitz
影响因子:
3.2
作者:
R. Kessin
通讯作者:
R. Kessin
影响因子:
3.6
作者:
P. Glaser;D. Ladant;O. Sezer;F. Pichot;A. Ullmann;A. Danchin
通讯作者:
A. Danchin