A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals.
A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals.
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DOI：
10.1038/ki.2011.294
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发表时间：
2012-01
影响因子：
19.6
通讯作者：
Molitoris BA
中科院分区：
文献类型：
--
作者：
Wang E;Meier DJ;Sandoval RM;Von Hendy-Willson VE;Pressler BM;Bunch RM;Alloosh M;Sturek MS;Schwartz GJ;Molitoris BA
肾小球滤过率（GFR）的测量是精确评估肾功能的金标准。GFR的快速、即时测定可在临床环境中提供优于当前可用测定的优势。在这里，我们在猪和狗中证明了这种方法的原理证明，这两个物种近似于人类预期的血管通路和GFR结果。在两种动物模型中，通过商业静脉内导管将递送激发光和收集荧光发射的亚毫米光纤插入外周静脉（狗）或中心静脉通路（猪）。一个小的自由可过滤的报告和大的非过滤血浆体积标记的荧光嵌合体的混合物注入作为一个团，激发发光二极管，并在体内信号检测和定量的光电倍增管在两个物种在不到60分钟。同时标准化6-碘海醇血浆肾脏清除率验证了我们的结果的准确性生理和慢性肾脏疾病设置。因此，我们的比率测量技术允许测量血浆血管容积和高度准确的实时GFR测定，从而能够真实的实时做出临床决策。
Measurement of the glomerular filtration rate (GFR) is the gold standard for precise assessment of kidney function. A rapid, point-of-care determination of the GFR may provide advantages in the clinical setting over currently available assays. Here we demonstrate a proof of principle for such an approach in a pig and dogs, two species that approximate the vascular access and GFR results expected in humans. In both animal models, a sub-millimeter optical fiber that delivered excitation light and collected fluorescent emissions was inserted into a peripheral vein (dog) or central venous access (pig) by means of commercial intravenous catheters. A mixture of fluorescent chimeras of a small freely filterable reporter and large non-filterable plasma volume marker were infused as a bolus, excited by light-emitting diodes, and the in vivo signals detected and quantified by photomultiplier tubes in both species in less than 60 min. Concurrent standardized 6-h iohexol plasma kidney clearances validated the accuracy of our results for both physiologic and a chronic kidney disease setting. Thus, our ratiometric technique allows for both measurement of plasma vascular volume and highly accurate real-time GFR determinations, enabling clinical decision making in real time.
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影响因子：
19.6
作者：
Schock-Kusch, Daniel;Xie, Qing;Gretz, Norbert
通讯作者：
Gretz, Norbert
影响因子：
--
作者：
SAPIRSTEIN, LA;VIDT, DG;HANUSEK, G
通讯作者：
HANUSEK, G
影响因子：
13.6
作者：
Ishani, Areef;Xue, Jay L.;Collins, Allan J.
通讯作者：
Collins, Allan J.
影响因子：
4.3
作者：
Zinderman, CE;Landow, L;Wise, RP
通讯作者：
Wise, RP
影响因子：
158.5
作者：
COLE, BR;GIANGIACOMO, J;INGELFINGER, JR
通讯作者：
INGELFINGER, JR