Analysis of the local organization and dynamics of cellular actin networks.
Analysis of the local organization and dynamics of cellular actin networks.
复制标题
DOI：
10.1083/jcb.201210123
复制
发表时间：
2013-09-30
期刊：
影响因子：
--
通讯作者：
Bershadsky AD
中科院分区：
文献类型：
--
作者：
Luo W;Yu CH;Lieu ZZ;Allard J;Mogilner A;Sheetz MP;Bershadsky AD
活细胞成像、高分辨率显微镜和计算模型显示，动态的形成-细丝-肌动蛋白自组织成肌球蛋白收缩网络，可能维持细胞质的机械连贯性。Ctin细丝在多种辅助蛋白的帮助下，自组装成各种网络模式。我们研究了连接纤维连接蛋白涂层粘附条的细胞的非粘附区中肌动蛋白网络的组织和动力学。该网络由肌球蛋白II结合并连接的肌动蛋白结点组成，含有形态发生的福尔马林紊乱相关激活剂1(DAAM1)和交联剂细丝素A(FLNA)。加入Latrunculin A(LATA)后，肌动蛋白结节更加突出，表现为漂移-扩散运动。超分辨显微镜显示，在未经处理的细胞中，DAAM1形成了与肌动蛋白结节类似的空间排列的斑块。LATA处理细胞中的节点运动(扩散系数和速度)依赖于肌球蛋白IIA、DAAM1和FLNA的水平和活性。基于我们的结果，我们开发了一个动态的形成-细丝-肌动蛋白网络的计算模型，该模型可以自组织成一个收缩的肌球蛋白网络。我们认为，这样的网络对于连接细胞的远端部分以维持细胞质的机械连贯性至关重要。
Live cell imaging, high-resolution microscopy, and computational modeling show that dynamic formin-filamin-actin asters self-organize into an actomyosin contractile network that may maintain mechanical coherence of cytoplasm. A ctin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. We studied the organization and dynamics of the actin network in nonadhesive regions of cells bridging fibronectin-coated adhesive strips. The network was formed by actin nodes associated with and linked by myosin II and containing the formin disheveled-associated activator of morphogenesis 1 (DAAM1) and the cross-linker filamin A (FlnA). After Latrunculin A (LatA) addition, actin nodes appeared to be more prominent and demonstrated drift-diffusion motion. Superresolution microscopy revealed that, in untreated cells, DAAM1 formed patches with a similar spatial arrangement to the actin nodes. Node movement (diffusion coefficient and velocity) in LatA-treated cells was dependent on the level and activity of myosin IIA, DAAM1, and FlnA. Based on our results, we developed a computational model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. We suggest that such networks are critical for connecting distant parts of the cell to maintain the mechanical coherence of the cytoplasm.
登录
查看更多内容
影响因子：
64.8
作者：
Martin, Adam C.;Kaschube, Matthias;Wieschaus, Eric F.
通讯作者：
Wieschaus, Eric F.
影响因子：
8.6
作者：
Dasanayake NL;Michalski PJ;Carlsson AE
通讯作者：
Carlsson AE
影响因子：
5.3
作者：
Li, Q;Lau, A;Stanley, EF
通讯作者：
Stanley, EF
影响因子：
3.5
作者：
Madrid, R;Gasteier, JE;Fackler, OT
通讯作者：
Fackler, OT
影响因子：
3.7
作者：
Ang, Su-Fen;Zhao, Zhuo-shen;Manser, Ed
通讯作者：
Manser, Ed