Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan.
Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan.
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DOI：
10.4142/jvs.2006.7.1.43
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发表时间：
2006-03
影响因子：
1.8
通讯作者：
Qureshi IZ
中科院分区：
文献类型：
--
作者：
Malik MW;Ayub N;Qureshi IZ
鸡传染性法氏囊病（Infectious bursal disease，IBD）是由Birna病毒引起的一种急性、高度接触性传染病。如果注射抗体，受感染鸟类的死亡率可以得到最好的预防。本研究试图在巴基斯坦提高特异性IBD病毒的超免疫多克隆抗体。将商品蛋鸡分为4组，按照4种不同的治疗方案皮下注射IBD疫苗。每天收集鸡蛋，用硫酸葡聚糖从蛋黄中纯化抗体。采用酶联免疫吸附试验和琼脂沉淀试验检测血清和卵黄抗体效价。卵黄中的抗体滴度显着高于血清。在接种后28天收集的鸡蛋具有最大抗体滴度。在治疗方案中，发现T3对超免疫最有效。冻干抗体在4℃下保存至实验结束仍保持活性。用纯化的抗体注射IBD病毒感染的鸟，与对照鸟相比，其诱导92%的回收率。该研究暗示纯化的抗体可用作治疗IBD感染的禽类的治疗剂。
Infectious bursal disease (IBD) is an acute and highly contagious disease of young chickens caused by Birnavirus. Mortality of infected birds can be best prevented if injected with antibodies. The present study was an attempt to raise specific hyper-immune polyclonal antibodies against IBD virus in Pakistan. Commercial layers divided into four groups were injected with IBD vaccine subcutaneously according to four different treatment regimens. Eggs were collected daily and antibodies were purified from yolk with dextran sulphate. Titers of antibodies in serum and yolk were evaluated with enzyme linked immunosorbant assay and agar gel precipitation test. Antibody titers were significantly higher in yolk than serum. Eggs collected at 28 days post-vaccination had maximum antibody titers. Of treatment regimens, T3 was found to be most effective for hyperimmunization. Lyophilized antibodies stored at 4℃ did not lose their activity till the end of experiment. IBD virus infected birds were injected with purified antibodies which induced 92% recovery as compared to control birds. The study implicates that the purified antibodies may be useful as a therapeutic agent to cure IBD infected birds.
影响因子：
2.9
作者：
STUART, CA;PIETRZYK, RA;GREEN, A
通讯作者：
GREEN, A
影响因子：
2.2
作者：
AKITA, EM;NAKAI, S
通讯作者：
NAKAI, S
影响因子：
3.9
作者：
AKITA, EM;NAKAI, S
通讯作者：
NAKAI, S