Effect of dentin biomodification delivered by experimental acidic and neutral primers on resin adhesion.
Effect of dentin biomodification delivered by experimental acidic and neutral primers on resin adhesion.
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DOI：
10.1016/j.jdent.2020.103354
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发表时间：
2020-08
影响因子：
4.4
通讯作者：
Bedran-Russo AK
中科院分区：
文献类型：
--
作者：
Trevelin LT;Alania Y;Mathew M;Phansalkar R;Chen SN;Pauli GF;Bedran-Russo AK
原花青素 (PAC) 是模仿天然胶原蛋白交联的生物化合物。任何生物化合物的有效且实用的递送对于临床使用至关重要。目的是研究两种富含 PAC 的高生物活性提取物（葡萄 (Vv) 和茶树 (Cs)）的牙本质生物改性和牙本质-树脂生物界面的有效形成，使用中性 (NP) 或酸性 (AP) 冲洗底漆方法进行递送。研究了牙本质脱矿深度（光学轮廓测定法）、牙本质生物改性（表观弹性模量、胶原蛋白自发荧光）和牙本质-树脂界面的特性（微拉伸粘合强度 - μTBS 和微渗透性）。 NP 由 15% Vv 或 Cs 组成，表面蚀刻后施加 60 秒；而 AP 则在 35% 乙醇酸 (GA) 或酒石酸 (TA) 中含有 15% Vv 或 Cs，施加 30 秒或 60 秒。使用方差分析和事后检验（α=0.05）对数据进行分析。无论采用何种冲洗底漆方法，使用 60 秒时，脱矿深度在统计上更高（p<0.001）。与 AP 策略相比，无论 PAC 提取物如何，NP 组都表现出统计上更高的表观弹性模量 (p < 0.001)。当应用 60 秒时，NPVv 获得了最高的 μTBS，与 APGAVv 在统计上相似（p<0.001）；两者均导致界面渗透率急剧下降。 NPC 组显示出最低的 μTBS (p<0.001)。使用乙醇酸与中高 PAC 低聚物富集提取物 (Vv) 可以实现高粘合强度和低微渗透性的组合。尽管表现出较高的初始牙本质生物改性，但主要含有儿茶素和二聚 PAC 的 Cs 提取物被发现不适合树脂-牙本质粘附。这项研究提供了 PAC 介导的牙本质生物改性和使用冲洗底漆的保守牙本质表面蚀刻的新概念。该策略需要 PAC 来源、α-羟基酸和施用时间的特定组合。
Proanthocyanidins (PACs) are biocompounds mimicking native collagen cross-links. The effective and practical delivery of any biocompound is pivotal for clinical usage. The aim was to investigate the dentin biomodification and effective formation of dentin-resin biointerfaces of two highly bioactive PAC-rich extracts, Vitis vinifera (Vv) and Camellia sinensis (Cs), delivered using neutral (NP) or acidic (AP) rinse-out primer approaches. The depth of dentin demineralization (optical profilometry), dentin biomodification (apparent modulus of elasticity, collagen auto-fluorescence) and properties of dentin-resin interfaces (microtensile bond strength – μTBS, and micro-permeability) were investigated. NP consisted of either 15% Vv or Cs applied for 60s after surface etching; while AP contained 15% Vv or Cs in either 35% glycolic acid (GA) or tartaric acid (TA) applied for 30s or 60s. Data were analyzed using ANOVA and post-hoc tests (α=0.05). The depth of demineralization was statistically higher when applied for 60s, regardless of rinse-out primer approach (p<0.001). Compared to AP strategy, NP groups exhibited statistically higher apparent modulus of elasticity, regardless of PAC extract (p < 0.001). Highest μTBS were obtained for NPVv, which were statistically similar to APGAVv, when applied for 60s (p<0.001); both resulted in a dramatic decrease of the interfacial permeability. NPCs group showed the lowest μTBS (p<0.001) A combination of high bond strength and low micro-permeability can be accomplished using glycolic acid with a mid- and high-PAC oligomer enriched extract (Vv). Cs extract containing mostly catechins and dimeric PACs, was found unsuitable for resin-dentin adhesion despite exhibiting high initial dentin biomodification. This study provides a new conceptual delivery of PAC-mediated dentin biomodification and conservative dentin surface etching using rinse-out primers. The strategy requires a specific combination of PAC source, α-hydroxyl acid, and application time.
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影响因子：
9.7
作者：
Vidal CM;Zhu W;Manohar S;Aydin B;Keiderling TA;Messersmith PB;Bedran-Russo AK
通讯作者：
Bedran-Russo AK
DOI：
10.1016/j.jmbbm.2011.05.003
发表时间：
2011-10
影响因子：
3.9
作者：
Castellan, Carina Strano;Bedran-Russo, Ana Karina;Karol, Sachin;Rodrigues Pereira, Patricia Nobrega
通讯作者：
Rodrigues Pereira, Patricia Nobrega
影响因子：
5
作者：
Zhou, Jianfeng;Chiba, Ayaka;Pashley, David H.
通讯作者：
Pashley, David H.
影响因子：
2.4
作者：
Leme, Ariene A.;Vidal, Cristina M. P.;Hassan, Lina Saleh;Bedran-Russo, Ana K.
通讯作者：
Bedran-Russo, Ana K.
影响因子：
5
作者：
Tezvergil-Mutluay, Arzu;Agee, Kelli A.;Hoshika, Tomohiro;Carrilho, Marcela;Breschi, Lorenzo;Tjaderhane, Leo;Nishitani, Yoshihiro;Carvalho, Ricardo M.;Looney, Stephen;Tay, Franklin R.;Pashley, David H.
通讯作者：
Pashley, David H.