Silver nanoparticles induce developmental stage-specific embryonic phenotypes in zebrafish.
Silver nanoparticles induce developmental stage-specific embryonic phenotypes in zebrafish.
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DOI：
10.1039/c3nr03210h
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发表时间：
2013-12-07
期刊：
影响因子：
6.7
通讯作者：
Xu XH
中科院分区：
文献类型：
--
作者：
Lee KJ;Browning LM;Nallathamby PD;Osgood CJ;Xu XH
人们对纳米材料在生物有机体中的开发和应用寄予厚望，但对其生物相容性和靶向特异性仍存在担忧。在这里，我们报告了我们的运输，生物相容性和毒性的纯化和稳定的银纳米粒子（银纳米粒子，直径为13.1 ± 2.5 nm）的斑马鱼胚胎的特定发育阶段，使用单NP等离子体光谱的研究。我们发现，单一的银纳米粒子被动扩散到五个不同的发育阶段的胚胎（卵裂，早期原肠胚，早期分割，后期分割，孵化阶段），显示阶段独立的扩散模式和扩散系数。值得注意的是，Ag NPs诱导独特的阶段和剂量依赖性表型和纳米毒性，其急性暴露于Ag NPs（0-0.7 nM）仅2小时。后期分割胚胎对具有最低临界浓度（CNP，c <0.02 nM）和最高心脏异常百分比的NP最敏感，其次是早期分割胚胎（CNP，c < 0.02 nM），这表明NP对细胞分化的破坏对胚胎发育造成最大的毒性作用。用NP处理的卵裂期胚胎发育为多种表型（异常鳍褶、尾/脊髓弯曲、心脏畸形、卵黄囊水肿和无头畸形）。这些器官结构在卵裂期胚胎中尚未发育，这表明创建这些结构的最早决定性事件正在进行中，并被NP破坏，这导致下游效应。相反，孵化胚胎对Ag NPs的抗性最强，大多数胚胎（94%）发育正常，没有一个胚胎发育异常。有趣的是，早期原肠胚胚胎对NP的敏感性低于卵裂和分割阶段胚胎，并且不会异常发育。这些重要的发现表明，Ag NPs不是简单的毒药，它们可以靶向发育中的特定途径，并可能使早期胚胎发育的靶向特异性研究和治疗成为可能。
Much is anticipated from the development and deployment of nanomaterials in biological organisms, but concerns remain regarding their biocompatibility and target specificity. Here we report our study of the transport, biocompatibility and toxicity of purified and stable silver nanoparticles (Ag NPs, 13.1 ± 2.5 nm in diameter) upon the specific developmental stages of zebrafish embryos using single NP plasmonic spectroscopy. We find that single Ag NPs passively diffuse into five different developmental stages of embryos (cleavage, early-gastrula, early-segmentation, late-segmentation, and hatching stages), showing stage-independent diffusion modes and diffusion coefficients. Notably, the Ag NPs induce distinctive stage and dose-dependent phenotypes and nanotoxicity, upon their acute exposure to the Ag NPs (0–0.7 nM) for only 2 h. The late-segmentation embryos are most sensitive to the NPs with the lowest critical concentration (CNP,c ≪ 0.02 nM) and highest percentages of cardiac abnormalities, followed by early-segmentation embryos (CNP,c < 0.02 nM), suggesting that disruption of cell differentiation by the NPs causes the most toxic effects on embryonic development. The cleavage-stage embryos treated with the NPs develop to a wide variety of phenotypes (abnormal finfold, tail/spinal cord flexure, cardiac malformation, yolk sac edema, and acephaly). These organ structures are not yet developed in cleavage-stage embryos, suggesting that the earliest determinative events to create these structures are ongoing, and disrupted by NPs, which leads to the downstream effects. In contrast, the hatching embryos are most resistant to the Ag NPs, and majority of embryos (94%) develop normally, and none of them develops abnormality. Interestingly, early-gastrula embryos are less sensitive to the NPs than cleavage and segmentation stage embryos, and do not develop abnormally. These important findings suggest that the Ag NPs are not simple poisons, and they can target specific pathways in development, and potentially enable target specific study and therapy for early embryonic development.
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影响因子：
17.1
作者：
Lee, Kerry J.;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy
影响因子：
3.4
作者：
KUSUMI, A;SAKO, Y;YAMAMOTO, M
通讯作者：
YAMAMOTO, M
影响因子：
6.7
作者：
Browning LM;Lee KJ;Huang T;Nallathamby PD;Lowman JE;Xu XH
通讯作者：
Xu XH
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
4.3
作者：
Lee, Kerry J.;Browning, Lauren M.;Huang, Tao;Ding, Feng;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy