Marine sediment-derived Streptomyces bacteria from British Columbia, Canada are a promising microbiota resource for the discovery of antimicrobial natural products.
Marine sediment-derived Streptomyces bacteria from British Columbia, Canada are a promising microbiota resource for the discovery of antimicrobial natural products.
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DOI：
10.1371/journal.pone.0077078
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Andersen RJ
中科院分区：
文献类型：
--
作者：
Dalisay DS;Williams DE;Wang XL;Centko R;Chen J;Andersen RJ
来自陆地来源的链霉菌属的代表在过去四十年中一直是深入研究的焦点，因为它们大量生产化学多样性和生物学重要的化合物。然而，在过去的几年里，由于重新发现了相同的生物活性化合物和样品菌株的冗余，来自这个生态位的代谢物研究显着下降。最近，一个新的画面已经开始出现，其中海洋来源的链霉菌细菌已经成为最新的热点，作为独特的和生物活性化合物的新来源。在这里，我们调查了海洋沉积物中收集的温带寒冷的沃茨从不列颠哥伦比亚省，加拿大作为一个有价值的来源，新的群体的海洋来源的链霉菌具有抗菌活性。从49个海洋沉积物样品中分离得到186株链霉菌，其中47株具有抗菌活性。活性菌株的系统发育分析导致在四个不同的集群的生物活性链霉菌，包括一个集群的菌株，似乎代表新的物种的鉴定。此外，我们探讨了这些海洋来源的链霉菌是否产生新的具有抗菌特性的次级代谢产物。化学分析揭示了结构多样的次级代谢产物，包括四种新的抗菌新生霉素类似物。我们对新生霉素类似物抗耐甲氧西林金黄色葡萄球菌（MRSA）进行了构效关系（SAR）研究。在这项研究中，我们揭示了新生二糖3”和4”位的氨基甲酰基和OMe部分以及羟基苯甲酸酯环5位的氢取代基对于抗MRSA活性的重要性。在这些位置的取代基的变化显着阻碍或完全消除新生霉素对MRSA的抑制活性。
Representatives of the genus Streptomyces from terrestrial sources have been the focus of intensive research for the last four decades because of their prolific production of chemically diverse and biologically important compounds. However, metabolite research from this ecological niche had declined significantly in the past years because of the rediscovery of the same bioactive compounds and redundancy of the sample strains. More recently, a new picture has begun to emerge in which marine-derived Streptomyces bacteria have become the latest hot spot as new source for unique and biologically active compounds. Here, we investigated the marine sediments collected in the temperate cold waters from British Columbia, Canada as a valuable source for new groups of marine-derived Streptomyces with antimicrobial activities. We performed culture dependent isolation from 49 marine sediments samples and obtained 186 Streptomyces isolates, 47 of which exhibited antimicrobial activities. Phylogenetic analyses of the active isolates resulted in the identification of four different clusters of bioactive Streptomyces including a cluster with isolates that appear to represent novel species. Moreover, we explored whether these marine-derived Streptomyces produce new secondary metabolites with antimicrobial properties. Chemical analyses revealed structurally diverse secondary metabolites, including four new antibacterial novobiocin analogues. We conducted structure-activity relationships (SAR) studies of these novobiocin analogues against methicillin-resistant Staphylococcus aureus (MRSA). In this study, we revealed the importance of carbamoyl and OMe moieties at positions 3” and 4” of novobiose as well as the hydrogen substituent at position 5 of hydroxybenzoate ring for the anti-MRSA activity. Changes in the substituents at these positions dramatically impede or completely eliminate the inhibitory activity of novobiocins against MRSA.
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影响因子：
16.6
作者：
Gulder, Tobias A. M.;Moore, Bradley S.
通讯作者：
Moore, Bradley S.
DOI：
10.1016/0021-9673(88)90007-6
发表时间：
1988-08-05
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ABIDI, SL
通讯作者：
ABIDI, SL
DOI：
10.1016/0005-2728(75)90173-5
发表时间：
1975-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GRIMMELIKHUIJZEN, CJP;MARRES, CAM;SLATER, EC
通讯作者：
SLATER, EC
DOI：
10.1023/a:1010296220929
发表时间：
2001-06-01
影响因子：
2.6
作者：
Egan, S;Wiener, P;Wellington, EMH
通讯作者：
Wellington, EMH
影响因子：
16.6
作者：
Feling, RH;Buchanan, GO;Fenical, W
通讯作者：
Fenical, W