Tailoring light delivery for optogenetics by modal demultiplexing in tapered optical fibers.
Tailoring light delivery for optogenetics by modal demultiplexing in tapered optical fibers.
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DOI：
10.1038/s41598-018-22790-z
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发表时间：
2018-03-13
影响因子：
4.6
通讯作者：
Pisanello F
中科院分区：
文献类型：
--
作者：
Pisanello M;Pisano F;Sileo L;Maglie E;Bellistri E;Spagnolo B;Mandelbaum G;Sabatini BL;De Vittorio M;Pisanello F
理想情况下，脑深部区域神经活动的光遗传控制需要使用非侵入性设备进行精确和灵活的光传输。为此，锥形光纤（TF）代表了一种通用工具，其可以在大的脑体积或空间受限的子区域上传递光，同时明显小于平裂光纤。在这项工作中，我们报告的可能性进一步延长发光长度沿着的锥形在0.4 mm-3.0 mm的范围内增加的数值孔径的TF NA = 0.66。我们研究了光的输入角（θin）与沿锥形沿着的输出位置之间的依赖关系，发现对于θin > 10°，这种关系是线性的。该锥形的分模解复用特性用光线跟踪模型来证实，并在准透明溶液和脑切片中表征473 nm和561 nm的光，其中两个波长用于仅使用一个波导同时照射脑的两个不同区域。本手稿中呈现的结果可以指导神经科学家基于这种模式分割多路分解方法设计他们的光遗传学实验，提供一种工具，可能允许动态靶向具有不同延伸的区域，从小鼠VTA到猕猴视觉皮层。
Optogenetic control of neural activity in deep brain regions ideally requires precise and flexible light delivery with non-invasive devices. To this end, Tapered Optical Fibers (TFs) represent a versatile tool that can deliver light over either large brain volumes or spatially confined sub-regions, while being sensibly smaller than flat-cleaved optical fibers. In this work, we report on the possibility of further extending light emission length along the taper in the range 0.4 mm-3.0 mm by increasing the numerical aperture of the TFs to NA = 0.66. We investigated the dependence between the input angle of light (θin) and the output position along the taper, finding that for θin > 10° this relationship is linear. This mode-division demultiplexing property of the taper was confirmed with a ray tracing model and characterized for 473 nm and 561 nm light in quasi-transparent solution and in brain slices, with the two wavelengths used to illuminate simultaneously two different regions of the brain using only one waveguide. The results presented in this manuscript can guide neuroscientists to design their optogenetic experiments on the base of this mode-division demultiplexing approach, providing a tool that potentially allow for dynamic targeting of regions with diverse extension, from the mouse VTA up to the macaque visual cortex.
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影响因子：
25
作者：
Pisanello F;Mandelbaum G;Pisanello M;Oldenburg IA;Sileo L;Markowitz JE;Peterson RE;Della Patria A;Haynes TM;Emara MS;Spagnolo B;Datta SR;De Vittorio M;Sabatini BL
通讯作者：
Sabatini BL
影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
影响因子：
4.6
作者：
Scharf R;Tsunematsu T;McAlinden N;Dawson MD;Sakata S;Mathieson K
通讯作者：
Mathieson K
DOI：
10.1073/pnas.1611769113
发表时间：
2016-12-13
影响因子：
11.1
作者：
Park, Sung Il;Shin, Gunchul;Rogers, John A.
通讯作者：
Rogers, John A.