HIV entry in macrophages is dependent on intact lipid rafts.
HIV entry in macrophages is dependent on intact lipid rafts.
复制标题
DOI：
10.1016/j.virol.2008.12.031
复制
发表时间：
2009-03-30
期刊：
影响因子：
3.7
通讯作者：
James W
中科院分区：
文献类型：
--
作者：
Carter GC;Bernstone L;Sangani D;Bee JW;Harder T;James W
巨噬细胞是HIV-1的重要天然靶细胞，但以前对病毒进入这些细胞的研究有限，膜胆固醇和脂筏的参与尚不清楚。胆固醇破坏巨噬细胞膜使用四种药理学试剂通过不同的机制：甲基-β环糊精，制霉菌素，菲律宾肽复合物和洛伐他汀，都显着抑制生产性HIV进入和逆转录。这些药物的抑制作用导致病毒从感染细胞中释放减少，并且可以通过加入水溶性胆固醇而基本上逆转。尽管HIV受体表达水平显著降低，但病毒同样与胆固醇破坏的细胞结合。发现巨噬细胞CD 4和CCR 5与具有典型的筏相关蛋白flotillin-1的耐洗涤剂膜分配。感染后早期观察到HIV颗粒与脂筏标记物（CTB-FITC）共定位。这些数据表明，巨噬细胞膜胆固醇是必不可少的艾滋病毒进入，并牵连脂筏参与。
Macrophages are an important natural target cell for HIV-1, but previous studies of virus entry into these cells are limited, and the involvement of membrane cholesterol and lipid rafts is unknown. Cholesterol disruption of macrophage membranes using four pharmacological agents acting by different mechanisms: methyl-β cyclodextrin, nystatin, filipin complex and Lovastatin, all significantly inhibited productive HIV entry and reverse transcription. The inhibitory effects of these drugs resulted in decreased virus release from infected cells, and could be substantially reversed by the addition of water-soluble cholesterol. The virus bound equally to cholesterol-disrupted cells even though HIV receptor expression levels were significantly reduced. Macrophage CD4 and CCR5 were found to partition with the detergent-resistant membranes with a typical raft-associating protein flotillin-1. HIV particles were observed co-localising with a marker of lipid rafts (CTB-FITC) early post infection. These data suggest that macrophage membrane cholesterol is essential for HIV entry, and implicate lipid raft involvement.
登录
查看更多内容
影响因子：
5.8
作者：
Li GM;Li YG;Yamate M;Li SM;Ikuta K
通讯作者：
Ikuta K
DOI：
10.1073/pnas.85.3.900
发表时间：
1988-02-01
影响因子：
11.1
作者：
ALOIA, RC;JENSEN, FC;GORDON, LM
通讯作者：
GORDON, LM
影响因子：
4.8
作者：
Cardaba, Clara Moyano;Kerr, Jason S.;Mueller, Anja
通讯作者：
Mueller, Anja
影响因子：
5.4
作者：
Chung, CS;Huang, CY;Chang, W
通讯作者：
Chang, W
影响因子：
5.4
作者：
Giguère, JF;Tremblay, MJ
通讯作者：
Tremblay, MJ