Myeloid cells migrate in response to IL-24.
Myeloid cells migrate in response to IL-24.
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DOI：
10.1016/j.cyto.2011.05.018
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发表时间：
2011-09
期刊：
影响因子：
3.8
通讯作者：
Howard, O. M. Zack
中科院分区：
文献类型：
--
作者：
Buzas, Krisztina;Oppenheim, Joost J.;Howard, O. M. Zack
IL-24（黑色素瘤分化相关基因7产物）是IL-10细胞因子家族的一员，据报道具有抗肿瘤活性。IL-24由免疫组织产生，可通过病原体相关分子诱导其在人外周血单核细胞中的表达。虽然已知免疫细胞会产生IL-24，但免疫细胞对IL-24的反应尚不清楚。利用重组人IL-24，我们证明了IL-24在体外诱导人单核细胞和中性粒细胞迁移。体内趋化模型显示IL-24可吸引CD11b阳性骨髓细胞。为了进一步表征IL-24的趋化反应和IL-24利用的受体类型，我们用信号通路抑制剂处理单核细胞。白介素-24诱导的迁移被百日咳毒素处理减少，从而暗示g蛋白偶联受体参与了这一过程。此外，MEK和JAK抑制剂显著降低单核细胞向IL-24的迁移。这些结果表明，IL-24激活免疫细胞中的几个信号级联反应，引发骨髓细胞的迁移，这可能有助于IL-24已知的抗癌作用。
IL-24 (melanoma differentiation associated gene 7 product) is a member of the IL-10 cytokine family that has been reported to possess anti-tumor activity. IL-24 is produced by immune tissues and its expression can be induced in human peripheral blood mononuclear cells by pathogen-associated molecules. While immune cells are known to produce IL-24, the response of immune cells to IL-24 is unclear. Using recombinant human IL-24, we demonstrated that IL-24 induces human monocyte and neutrophil migration, in vitro. An in vivo chemotaxis model showed that IL-24 attracted CD11b positive myeloid cells. To further characterize the chemotactic IL-24 response and type(s) of receptor(s) utilized by IL-24, we treated monocytes with signaling pathway inhibitors. IL-24-induced migration was reduced by pertussis toxin treatment, thus implicating G-protein coupled receptors in this process. Additionally, MEK and JAK inhibitors markedly decreased monocyte migration toward IL-24. These results suggest that IL-24 activates several signaling cascades in immune cells eliciting migration of myeloid cells, which may contribute to the known anti-cancer effects of IL-24.
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DOI：
10.1073/pnas.152327199
发表时间：
2002-07-23
影响因子：
11.1
作者：
Sarkar, D;Su, ZZ;Fisher, PB
通讯作者：
Fisher, PB
影响因子：
5
作者：
Hsieh, MY;Chen, WY;Chang, MS
通讯作者：
Chang, MS
影响因子：
20.3
作者：
Balabanian, K;Foussat, A;Emilie, D
通讯作者：
Emilie, D
影响因子：
4.4
作者：
Caudell, EG;Mumm, JB;Grimm, EA
通讯作者：
Grimm, EA
影响因子：
1.5
作者：
Buzas, Krisztina;Megyeri, Klara
通讯作者：
Megyeri, Klara