Proteomics analysis of perilymph and cerebrospinal fluid in mouse.
Proteomics analysis of perilymph and cerebrospinal fluid in mouse.
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DOI：
10.1002/lary.20209
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发表时间：
2009-05
期刊：
影响因子：
2.6
通讯作者：
Sewell, William F.
中科院分区：
文献类型：
--
作者：
Swan, Erin E. Leary;Peppi, Marcello;Chen, Zhiqiang;Green, Karin M.;Evans, James E.;McKenna, Michael J.;Mescher, Mark J.;Kujawa, Sharon G.;Sewell, William F.
外淋巴液中的蛋白质可能通过生物污染改变植入式脑内药物递送系统的递送特性。蛋白质组成的知识将有助于预测与递送的药剂的相互作用。小鼠外淋巴液的分析。采用基于毛细管液相色谱-质谱的iTRAQ定量蛋白质组学方法分析外淋巴液和脑脊液（CSF）的蛋白质组成。我们搜索了Uniprot FASTA蛋白质数据库的小鼠子集。我们从小鼠耳蜗顶端采集外淋巴液，以尽量减少CSF污染。以非常高的置信度鉴定了超过50种明确的蛋白质同种型。iTRAQ报告离子允许测定外淋巴液和CSF之间蛋白质的相对摩尔量。外淋巴液中的蛋白质浓度几乎是脑脊液中的3倍。外淋巴中超过三分之一的蛋白质包含蛋白酶抑制剂，其中丝氨酸蛋白酶抑制剂是主要的组。载脂蛋白类占16%。15%的蛋白质是酶。白蛋白是最丰富的单一蛋白质（14%）。具有相对高的外淋巴/CSF比率的蛋白质包括广谱蛋白酶抑制剂和载脂蛋白。在外淋巴液中发现的一些蛋白质，如白蛋白和HMW激肽原，通过吸附到器械材料而与生物污垢有关。相对大量的载脂蛋白和白蛋白可作为酸性和亲脂性药物的储库。α-2-糖蛋白可以结合碱性药物。外淋巴液的蛋白质组成与脑脊液相似，但含量高出2.8倍。蛋白酶抑制剂包括最大类别的蛋白质。
Proteins in perilymph may alter the delivery profile of implantable intracochlear drug delivery systems through biofouling. Knowledge of protein composition will help anticipate interactions with delivered agents. Analysis of mouse perilymph. Protein composition of perilymph and cerebrospinal fluid (CSF) was analyzed using a capillary liquid chromatography-mass spectrometry-based iTRAQ quantitative proteomics approach. We searched against a mouse subset of the Uniprot FASTA protein database. We sampled perilymph from the apex of the mouse cochlea to minimize CSF contamination. More than 50 explicit protein isoforms were identified with very high confidence. iTRAQ reporter ions allowed determination of relative molar amounts of proteins between perilymph and CSF. Protein in perilymph was almost three times more concentrated than in CSF. More than one-third of the proteins in perilymph comprised protease inhibitors, with serpins being the predominant group. Apolipoproteins constituted 16%. Fifteen percent of the proteins were enzymes. Albumin was the most abundant single protein (14%). Proteins with relatively high perilymph/CSF ratios included broad-spectrum protease inhibitors and apolipoproteins. Some proteins found in perilymph, such as albumin and HMW kininogen, have been implicated in biofouling through adsorption to device materials. The relatively large quantities of apolipoprotein and albumin may serve as a reservoir for acidic and lipophillic drugs. Alpha-2-glycoprotein can bind basic drugs. Perilymph is similar in protein composition to CSF, though amounts are 2.8 times higher. Protease inhibitors comprise the largest category of proteins.
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影响因子：
2.9
作者：
BROWNE, CA;BENNETT, HPJ;SOLOMON, S
通讯作者：
SOLOMON, S
影响因子：
16.1
作者：
Swan, Erin E. Leary;Mescher, Mark J.;Sewell, William F.;Tao, Sarah L.;Borenstein, Jeffrey I.
通讯作者：
Borenstein, Jeffrey I.
影响因子：
7
作者：
Ross, PL;Huang, YLN;Pappin, DJ
通讯作者：
Pappin, DJ
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
影响因子：
13.5
作者：
Lai, Keane K. Y.;Kolippakkam, Deepak;Beretta, Laura
通讯作者：
Beretta, Laura