Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.
Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.
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盐胁迫通过调节菊芋植株中脯氨酸的生物合成和降解来促进脯氨酸积累
DOI：
10.1371/journal.pone.0062085
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Long X
中科院分区：
文献类型：
--
作者：
Huang Z;Zhao L;Chen D;Liang M;Liu Z;Shao H;Long X
脯氨酸积累是盐胁迫下渗透调节的一种重要机制。在本研究中，我们评估了菊芋（Helianthus tuberosus L.）幼苗在氯化钠胁迫下根、茎和叶中的脯氨酸积累情况。我们还检测了HtP5CS、HtOAT和HtPDH酶的活性以及假定的HtP5CS1、HtP5CS2、HtOAT、HtPDH1和HtPDH2基因的表达模式。我们研究的目的是阐明菊芋（一种中度耐盐物种）在氯化钠胁迫下的脯氨酸调节机制。观察到菊芋幼苗在盐胁迫期间在根、茎和叶中积累脯氨酸。在氯化钠胁迫下，HtP5CS酶活性增加，而HtOAT和HtPDH活性普遍受到抑制。响应氯化钠胁迫，HtP5CS2的转录水平增加，而HtOAT、HtPDH1和HtPDH2的转录水平普遍降低。我们的研究结果支持，对于菊芋而言，盐胁迫下的脯氨酸合成主要通过谷氨酸途径，并且在这个过程中HtP5CS2起主要作用，而HtOAT的作用不太重要。两个HtPDH基因可能在脯氨酸降解中起作用。
Proline accumulation is an important mechanism for osmotic regulation under salt stress. In this study, we evaluated proline accumulation profiles in roots, stems and leaves of Jerusalem artichoke (Helianthus tuberosus L.) plantlets under NaCl stress. We also examined HtP5CS, HtOAT and HtPDH enzyme activities and gene expression patterns of putative HtP5CS1, HtP5CS2, HtOAT, HtPDH1, and HtPDH2 genes. The objective of our study was to characterize the proline regulation mechanisms of Jerusalem artichoke, a moderately salt tolerant species, under NaCl stress. Jerusalem artichoke plantlets were observed to accumulate proline in roots, stems and leaves during salt stress. HtP5CS enzyme activities were increased under NaCl stress, while HtOAT and HtPDH activities generally repressed. Transcript levels of HtP5CS2 increased while transcript levels of HtOAT, HtPDH1 and HtPDH2 generally decreased in response to NaCl stress. Our results supports that for Jerusalem artichoke, proline synthesis under salt stress is mainly through the Glu pathway, and HtP5CS2 is predominant in this process while HtOAT plays a less important role. Both HtPDH genes may function in proline degradation.
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影响因子：
1.6
作者：
Kimura, T;Nakano, T;Yoshida, S
通讯作者：
Yoshida, S
影响因子：
4.3
作者：
Almeida da Rocha, Iza Marineves;Vitorello, Victor Alexandre;Gomes Silveira, Joaquim Albenisio
通讯作者：
Gomes Silveira, Joaquim Albenisio
DOI：
10.1016/0144-4565(84)90066-0
发表时间：
1984-01-01
期刊：
BIOMASS
影响因子：
--
作者：
KOSARIC, N;COSENTINO, GP;DUVNJAK, Z
通讯作者：
DUVNJAK, Z
影响因子：
3
作者：
NEWTON, PJ;MYERS, BA;WEST, DW
通讯作者：
WEST, DW
影响因子：
4.3
作者：
Gruszka Vendruscolo, Eliane Cristina;Schuster, Ivan;Esteves Vieira, Luiz Gonzaga
通讯作者：
Esteves Vieira, Luiz Gonzaga