Moesin and its activating kinase Slik are required for cortical stability and microtubule organization in mitotic cells.
Moesin and its activating kinase Slik are required for cortical stability and microtubule organization in mitotic cells.
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DOI：
10.1083/jcb.200709161
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发表时间：
2008-02-25
期刊：
影响因子：
--
通讯作者：
Payre F
中科院分区：
文献类型：
--
作者：
Carreno S;Kouranti I;Glusman ES;Fuller MT;Echard A;Payre F
细胞分裂需要涉及皮质肌动蛋白局部重组的细胞形状改变，这必须与有丝分裂纺锤体操作的染色体分离紧密相关。这个多步骤过程是如何协调的，人们仍然知之甚少。在这项研究中，我们发现了肌动蛋白/膜连接蛋白moesin，即黑皮肤果蝇的单一ERM （ezrin， radixin和moesin）蛋白，在有丝分裂期间维持皮层稳定性是必需的。有丝分裂开始的特点是由丝激酶依赖性磷酸化介导的蛋白激活爆发。激活的moesin在早期中期均匀地定位于皮层，并在后期逐渐限制在赤道。肌动球蛋白的缺乏或抑制其激活会在有丝分裂过程中破坏皮质的稳定，导致严重的皮质变形和肌动球蛋白调节因子的异常分布。抑制moesin激活也会损害微管组织，并妨碍有丝分裂纺锤体的稳定定位。我们提出，有丝分裂皮层中moesin激活的时空控制为协调细胞分裂过程中皮质收缩性和微管相互作用提供了局部线索。
Cell division requires cell shape changes involving the localized reorganization of cortical actin, which must be tightly linked with chromosome segregation operated by the mitotic spindle. How this multistep process is coordinated remains poorly understood. In this study, we show that the actin/membrane linker moesin, the single ERM (ezrin, radixin, and moesin) protein in Drosophila melanogaster, is required to maintain cortical stability during mitosis. Mitosis onset is characterized by a burst of moesin activation mediated by a Slik kinase–dependent phosphorylation. Activated moesin homogenously localizes at the cortex in prometaphase and is progressively restricted at the equator in later stages. Lack of moesin or inhibition of its activation destabilized the cortex throughout mitosis, resulting in severe cortical deformations and abnormal distribution of actomyosin regulators. Inhibiting moesin activation also impaired microtubule organization and precluded stable positioning of the mitotic spindle. We propose that the spatiotemporal control of moesin activation at the mitotic cortex provides localized cues to coordinate cortical contractility and microtubule interactions during cell division.
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影响因子：
7.5
作者：
Hughes, Sarah C.;Fehon, Richard G.
通讯作者：
Fehon, Richard G.
DOI：
10.1083/jcb.200207130
发表时间：
2003-01-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Maddox AS;Burridge K
通讯作者：
Burridge K
影响因子：
9.2
作者：
Hickson, GRX;Echard, A;O'Farrell, PH
通讯作者：
O'Farrell, PH
影响因子：
64.8
作者：
Speck, O;Hughes, SC;Fehon, RG
通讯作者：
Fehon, RG
影响因子：
3.3
作者：
Giansanti, MG;Farkas, RM;Gatti, M
通讯作者：
Gatti, M