Multiple insecticide resistance mechanisms in Anopheles gambiae s.l. populations from Cameroon, Central Africa.
Multiple insecticide resistance mechanisms in Anopheles gambiae s.l. populations from Cameroon, Central Africa.
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DOI：
10.1186/1756-3305-6-41
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发表时间：
2013-02-22
影响因子：
3.2
通讯作者：
Simard F
中科院分区：
文献类型：
--
作者：
Nwane P;Etang J;Chouaїbou M;Toto JC;Koffi A;Mimpfoundi R;Simard F
按蚊对滴滴涕和拟除虫菊酯类杀虫剂耐药性的增加被视为控制疟疾病媒的一个限制因素。本研究旨在深入表征安。冈比亚血吸虫对喀麦隆杀虫剂的耐药性，以指导疟疾病媒控制干预措施。在喀麦隆4个主要生物地理域的6个地点收集冈比亚按蚊幼虫和蛹，在昆虫饲养场饲养至成虫。采用4%滴滴涕、0.75%氯菊酯和0.05%溴氰菊酯进行世卫组织标准药敏试验。测定了死亡率和敲除时间（kdt50和kdt95），并评估了预接触增效剂DEF、DEM和PBO的效果。采用聚合酶链反应- rflp法鉴定蚊虫种类和分子形态（M或S）。使用热结扎法描述kdr突变，并进行生化测定以评估解毒酶的活性。一个。对DDT和氯菊酯完全敏感（死亡率约为98%），对溴氰菊酯敏感性较低。对滴滴涕的抗药性在中国很普遍。观察了不同种群对氯菊酯和溴氰菊酯的不同敏感程度。在许多情况下，事先接触增效剂在一定程度上恢复了杀虫剂的击倒效果，并增加了死亡率，这表明解毒酶在提高蚊子在受拟除虫菊酯攻击后的存活率方面发挥了作用，在较低程度上也提高了滴滴涕的存活率。kdr等位基因的分布表明，kdr基抗性在S型安虫中起主要作用。冈比亚按蚊。在生化试验中，除一种蚊子种群外，其余蚊子种群均过度表达P450活性，而在所有野外蚊子种群和对照中，GST的基线活性均较低且相似。在喀麦隆，多种耐药机制以S型An分离。冈比亚菌产生异质抗性谱，而在M型和An。Arabiensis对杀虫剂的耐受性似乎主要是由酶解毒介导的。增效剂部分恢复了对拟除虫菊酯类杀虫剂的敏感性，并可能有助于减轻田间媒介抗药性的影响。然而，需要更多的病媒控制工具来进一步影响这种环境中的疟疾传播。
Increasing incidence of DDT and pyrethroid resistance in Anopheles mosquitoes is seen as a limiting factor for malaria vector control. The current study aimed at an in-depth characterization of An. gambiae s.l. resistance to insecticides in Cameroon, in order to guide malaria vector control interventions. Anopheles gambiae s.l. mosquitoes were collected as larvae and pupae from six localities spread throughout the four main biogeographical domains of Cameroon and reared to adults in insectaries. Standard WHO insecticide susceptibility tests were carried out with 4% DDT, 0.75% permethrin and 0.05% deltamethrin. Mortality rates and knockdown times (kdt50 and kdt95) were determined and the effect of pre-exposure to the synergists DEF, DEM and PBO was assessed. Tested mosquitoes were identified to species and molecular forms (M or S) using PCR-RFLP. The hot ligation method was used to depict kdr mutations and biochemical assays were conducted to assess detoxifying enzyme activities. The An. arabiensis population from Pitoa was fully susceptible to DDT and permethrin (mortality rates > 98%) and showed reduced susceptibility to deltamethrin. Resistance to DDT was widespread in An. gambiae s.s. populations and heterogeneous levels of susceptibility to permethrin and deltamethrin were observed. In many cases, prior exposure to synergists partially restored insecticide knockdown effect and increased mortality rates, suggesting a role of detoxifying enzymes in increasing mosquito survival upon challenge by pyrethroids and, to a lower extent DDT. The distribution of kdr alleles suggested a major role of kdr-based resistance in the S form of An. gambiae. In biochemical tests, all but one mosquito population overexpressed P450 activity, whereas baseline GST activity was low and similar in all field mosquito populations and in the control. In Cameroon, multiple resistance mechanisms segregate in the S form of An. gambiae resulting in heterogeneous resistance profiles, whereas in the M form and An. arabiensis insecticide tolerance seems to be essentially mediated by enzyme-based detoxification. Synergists partially restored susceptibility to pyrethroid insecticides, and might help mitigate the impact of vector resistance in the field. However, additional vector control tools are needed to further impact on malaria transmission in such settings.
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影响因子：
0.6
作者：
Cakir, G.;Yavuz, O.;Kocak, O.
通讯作者：
Kocak, O.
影响因子：
3
作者：
Etang J;Nwane P;Mbida JA;Piameu M;Manga B;Souop D;Awono-Ambene P
通讯作者：
Awono-Ambene P
影响因子：
1.7
作者：
Braks, Marieta A. H.;Carde, Ring T.
通讯作者：
Carde, Ring T.
影响因子：
3.3
作者：
Chung, Henry;Bogwitz, Michael R.;Daborn, Phillip J.
通讯作者：
Daborn, Phillip J.
影响因子：
3
作者：
Antonio-Nkondjio, Christophe;Fossog, Billy Tene;Ranson, Hilary
通讯作者：
Ranson, Hilary