Over-expression of the AtGA2ox8 gene decreases the biomass accumulation and lignification in rapeseed (Brassica napus L.)
Over-expression of the AtGA2ox8 gene decreases the biomass accumulation and lignification in rapeseed (Brassica napus L.)
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AtGA2ox8 基因的过度表达会降低油菜 (Brassica napus L.) 的生物量积累和木质化
DOI：
10.1631/jzus.b1000161
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发表时间：
2010-07
影响因子：
5.1
通讯作者：
Li, Ming-fang
中科院分区：
文献类型：
--
作者：
Huang, Xing-qun;Peng, Dan;He, Re-qing;Peng, Wu-sheng;Zhou, Bo;Zhu, Deng-feng;Lin, Jian-zhong;Zhuo, Yu-hong;Tang, Dong-ying;Liu, Xuan-ming;Zhao, Xiao-ying;Li, Ming-fang
赤霉素2-氧化酶（GA 2-oxidase，GA 2-oxidase）在植物生长发育中起着重要作用.本研究将拟南芥AtGA 2 ox 8基因转化油菜，并在油菜中过量表达。评估AtGA 2 ox 8在植物生物量积累和木质化中的作用。通过抗性筛选、聚合酶链反应（PCR）和逆转录PCR（RT-PCR）分析以及绿色荧光检测，对转基因植株进行了鉴定，结果表明，转基因植株生长迟缓、开花延迟、植株矮小。转基因株系的鲜重和干重分别比野生型低21%和29%，鲜干重比高于野生型。定量测定表明，转基因株系中木质素含量降低了10%-20%，组织化学染色结果也表明转基因株系中木质化减少。实时荧光定量PCR分析表明，转基因株系中木质素生物合成基因的转录水平显著降低，这与木质化的减少是一致的。这些结果表明，AtGA 2 ox 8过表达油菜中生物量积累和木质化的减少可能是由于木质素生物合成基因表达的改变。
Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. The transgenic plants, identified by resistant selection, polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) analyses, and green fluorescence examination, showed growth retardation, flowering delay, and dwarf stature. The fresh weight and dry weight in transgenic lines were about 21% and 29% lower than those in wild type (WT), respectively, and the fresh to dry weight ratios were higher than that of WT. Quantitative measurements demonstrated that the lignin content in transgenic lines decreased by 10%–20%, and histochemical staining results also showed reduced lignification in transgenic lines. Quantitative real-time PCR analysis indicated that the transcript levels of lignin biosynthetic genes in transgenic lines were markedly decreased and were consistent with the reduced lignification. These results suggest that the reduced biomass accumulation and lignification in the AtGA2ox8 overexpression rapeseed might be due to altered lignin biosynthetic gene expression.
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DOI：
10.1073/pnas.95.5.2686
发表时间：
1998-03-03
影响因子：
11.1
作者：
Lin, CT;Yang, HY;Cashmore, AR
通讯作者：
Cashmore, AR
影响因子：
2.9
作者：
Qiming Wang;Xiaoju Tu;Keqin Deng;Jianxin Zeng;Xiaoying Zhao;Dong-Ying Tang;Xuanming Liu
通讯作者：
Qiming Wang;Xiaoju Tu;Keqin Deng;Jianxin Zeng;Xiaoying Zhao;Dong-Ying Tang;Xuanming Liu
影响因子：
6.9
作者：
Appleford, Nigel E. J.;Wilkinson, Mark D.;Lenton, John R.
通讯作者：
Lenton, John R.
影响因子：
3.3
作者：
R. S. Fukushima;B. A. Dehority
通讯作者：
R. S. Fukushima;B. A. Dehority
影响因子：
4
作者：
A. L. Koch;D. White
通讯作者：
A. L. Koch;D. White