Transcriptomic analyses during the transition from biomass production to lipid accumulation in the oleaginous yeast Yarrowia lipolytica.
Transcriptomic analyses during the transition from biomass production to lipid accumulation in the oleaginous yeast Yarrowia lipolytica.
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DOI：
10.1371/journal.pone.0027966
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Nicaud JM
中科院分区：
文献类型：
--
作者：
Morin N;Cescut J;Beopoulos A;Lelandais G;Le Berre V;Uribelarrea JL;Molina-Jouve C;Nicaud JM
我们以前开发了一种用于产油酵母Y中脂质积累的发酵方案。解脂这个过程被用来进行转录时程分析，以探索基因表达的Y。在从生物质生产到脂质积累的过渡期间，在该实验中，在约10000000的条件下获得了54.6gCDW/l的生物质浓度和0.18g/gCDW脂质。32 h，柠檬酸产量较低。在整个发酵过程中对11个样品进行转录组学分析。通过统计分析，在实验的时间过程中，569个基因被突出显示为在一个点上差异表达。根据基因表达谱将这些基因分为9类。宏观和转录组学特征的组合突出了培养中的4个主要步骤：（i）生长阶段，（ii）过渡阶段，（iii）早期脂质积累阶段，其特征在于氮代谢的增加，以及蛋白质产生和活性的强烈抑制;（iv）晚期脂质积累阶段，其特征在于细胞内碳通量的重新路由。本研究探讨了Y.通过在转录组水平上鉴定可能参与产油物种代谢的基因，将解脂菌作为替代产油者。
We previously developed a fermentation protocol for lipid accumulation in the oleaginous yeast Y. lipolytica. This process was used to perform transcriptomic time-course analyses to explore gene expression in Y. lipolytica during the transition from biomass production to lipid accumulation. In this experiment, a biomass concentration of 54.6 gCDW/l, with 0.18 g/gCDW lipid was obtained in ca. 32 h, with low citric acid production. A transcriptomic profiling was performed on 11 samples throughout the fermentation. Through statistical analyses, 569 genes were highlighted as differentially expressed at one point during the time course of the experiment. These genes were classified into 9 clusters, according to their expression profiles. The combination of macroscopic and transcriptomic profiles highlighted 4 major steps in the culture: (i) a growth phase, (ii) a transition phase, (iii) an early lipid accumulation phase, characterized by an increase in nitrogen metabolism, together with strong repression of protein production and activity; (iv) a late lipid accumulation phase, characterized by the rerouting of carbon fluxes within cells. This study explores the potential of Y. lipolytica as an alternative oil producer, by identifying, at the transcriptomic level, the genes potentially involved in the metabolism of oleaginous species.
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DOI：
10.1016/j.bioeng.2007.04.003
发表时间：
2007-06-01
期刊：
BIOMOLECULAR ENGINEERING
影响因子：
--
作者：
Bras, Ligia P.;Menezes, Jose C.
通讯作者：
Menezes, Jose C.
影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL
影响因子：
4.4
作者：
Beopoulos, Athanasios;Mrozova, Zuzana;Nicaud, Jean-Marc
通讯作者：
Nicaud, Jean-Marc
影响因子：
64.8
作者：
DRAPKIN, R;REARDON, JT;REINBERG, D
通讯作者：
REINBERG, D
影响因子：
5.6
作者：
DUBOIS, E;HIERNAUX, D;WIAME, JM
通讯作者：
WIAME, JM