Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.

Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.
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DOI:
10.1085/jgp.68.2.197
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发表时间:
1976-08
期刊:
The Journal of general physiology
影响因子:
--
通讯作者:
Smock T
Smock T
中科院分区:
其他
文献类型:
--
作者:
Arch S;Earley P;Smock T

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It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.
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发表时间: 1972-01-01
影响因子: 3.8
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期刊: JOURNAL OF NEUROBIOLOGY
影响因子: --
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